13 research outputs found

    Differentially expressed salivary gland proteins between the susceptible Kis and resistant AceRKis strains.

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    <p>Protein identification was performed using MALDI-TOF MS or nanoLC ESI MS/MS (underlined spot#) analysis. Insecta entries of Swiss-Prot and TrEMBL databases were searched by using the MASCOT algorithm, or the Proteome Discoverer software for nanoLC ESI MS/MS spectra. All spots displayed a power >0.9. Spot# refers to the SameSpots analysis spot number (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103816#pone-0103816-g001" target="_blank">Figure 1A</a>); <sup>SP</sup> indicates the presence of a signal peptide for secretion as predicted by SignalP 4.0 (<i><sup>SP°</sup></i>: SP in the N-terminal homolog Q5TV62_ANOGA); Fold: fold difference in expression levels between the two strains; Accession: accession number in UniProtKB/Swiss-Prot or UniProtKB/TrEMBL databases (_ANOGA); pI: isoelectric point; Cover: indicates the amino acid coverage (%); *Mascot Score is provided for MALDI-TOF protein identification; for nanoLC ESI MS/MS based identification the peptide sequences and the number of peptides are provided as supporting information (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103816#pone.0103816.s001" target="_blank">Table S1</a>). Translation IF: Translation Initiation Factor.</p

    Differential expression of the salivary proteins between the susceptible Kis and resistant AceRKis SGE extracts.

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    <p>Differences in protein expression are represented as a function of both expression ratio (resistant/susceptible) and significance ratio (q-value). Vertical dotted lines indicate the 1.5-fold difference in expression level in either direction (x1.5 for a higher expression in the resistant strain and/1.5 for a lower expression in the resistant strain). The horizontal dotted line indicates a q-value  = 0.05 (or 1/q  = 20). Saglin and TRIO proteins display expression level differences above 3 and highly significant q-values.</p

    Amino-acid sequence of gSG6 Peptides.

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    <p>Amino-acid sequence of the SG6 protein of <i>Anopheles gambiae</i> (gi:13537666) is presented and sequences of the selected peptides, gSG6-P1 to gSG6-P5, are underlined. Signal peptide (SP) sequence is indicating by dotted underline.</p
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