Photograph showing thoracic vertebra trauma.

<p>Photograph showing thoracic vertebra trauma.</p

Photograph showing irregular bony outgrowths on both sides of the frontal crest.

<p>Photograph showing irregular bony outgrowths on both sides of the frontal crest.</p

Pyrosequencing results for a part of the amelogenin gene for sex determination.

<p>The upper pyrogram (skull sample) indicates a female individual, which can be seen by the different sequence pattern from dispensation 19 to 23 due to the six-bp deletion female individuals have in this part of the amelogenin gene. The lower pyrogram (the reference material) shows a male individual.</p

Primer sequences and cycling conditions used for amplification.

<p>Primer sequences and cycling conditions used for amplification.</p

Schedule showing preserved elements (in black).

<p>Schedule showing preserved elements (in black).</p

STR genotypes and mtDNA determined in the remains and the reference sample.

<p>Frequency* - allele frequencies determined in the Swedish population (Divne et al. 2010).</p><p>LR** - Likelihood ratios.</p

Bone preservation and measurements.

<p>(GL = greatest length, ant-post = anterior-posterior diameter, inf-sup = inferior-superior diameter, med-lat = medial-lateral diameter, Max Mid = maximal diameter at midshaft, Min Mid = minimal diameter at midshaft, GdB = greatest distal breadth).</p

Results of mtDNA Sanger sequencing, Pyrosequencing and age determination.

1<p>rCRS, revised Cambridge Reference Sequence <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008986#pone.0008986-Andrews1" target="_blank">[33]</a>. Skull A, sequence from the putative skull of St. Birgitta 1303–1373. Skull B, sequence from the putative skull of Katarina 1331–1381. Analyst 1, sequence from Analyst 1. Analyst 2, sequence from Analyst 2.</p>2<p>Pyrosequencing results of mtDNA coding regions.</p>3<p>Mean value of three different sample preparations from two different bone samples from the skull A.</p>4<p>σ refers to the standard deviation, where 1σ corresponds to 68.2% probability and 2σ to 95.4% probability according to the OxCal v3.10.</p

Picture of the relics.

<p>The putative skull of St. Birgitta (skull A) to the right and the putative skull of Katarina (skull B) to the left. (Photograph: Hans Lundberg).</p

Primer sequences and cycling conditions used for amplification.

1<p>Amplification was performed in a GeneAmp 9700 PCR System (Applied Biosystems) by a 10 min incubation at 95°C, followed by 45 cycles of 301 s. at 95°C, 45 s. at 60°C and 60 s. at 72°C. The program was completed by an extension step at 72°C for 7 min and a final hold at 4°C.</p>2<p>Amplification of the coding mtDNA fragments was performed as described above, with an annealing temperature of 53°C instead of 60°C <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008986#pone.0008986-Andreasson1" target="_blank">[10]</a>.</p>3<p>Amplification of the amelogenin gene was performed in a GeneAmp 9700 PCR System (Applied Biosystems). The cycling conditions were 10 minutes at 95°C, 45 cycles of 30 s at 95°C, 45 s at 55°C, 60 s at 72°C and a final extension step for 7 minutes at 72°C.</p