IgE levels against α-Gal at the three different time points (at diagnosis=“0 months”; after two to three months and six months) among the 36 α-Gal IgE positive erythema migrans patients.

<p>The levels were compared between the different time points. A significant reduction in IgE anti-α-Gal levels from both the first and second sample to the third sample (p<0.01).</p

Basic clinical data on blood donors according to IgE anti-α-Gal status.

<p>Basic clinical data on blood donors according to IgE anti-α-Gal status.</p

Dual immunostaining of anti-tryptase and -chymase in the same mast cell located within the interior wall of right ventricle

The insert: Cardiac mast cell staining only with anti-tryptase, MC. () Mast cell degranulation in the heart section of a drug victim. Original magnification × 400, in A and × 200 in b.<p><b>Copyright information:</b></p><p>Taken from "Pathogenic role of cardiac mast cell activation/degranulation, TNF-α, and cell death in acute drug-related fatalities"</p><p></p><p>Vascular Health and Risk Management 2007;3(6):1053-1062.</p><p>Published online Jan 2007</p><p>PMCID:PMC2350147.</p><p>© 2007 Perskvist et al, publisher and licensee Dove Medical Press Ltd.</p

Correlation between total IgE and IgE responses to α-Gal in erythema migrans patients (n = 27).

<p>Correlation between total IgE and IgE responses to α-Gal in erythema migrans patients (n = 27).</p

IgE anti-α-Gal positive, total IgE and Phadiatop status in erythema migrans patients.

<p>IgE anti-α-Gal positive, total IgE and Phadiatop status in erythema migrans patients.</p

IgE anti-α-Gal positive rate in blood donors according to <i>Borrelia</i> status.

<p>IgE anti-α-Gal positive rate in blood donors according to <i>Borrelia</i> status.</p

TUNEL-positive myocytes nuclei (brown) demonstrated with the ApopTag kit

The transverse section is from the left ventricle in a case of SND (× 200 magnifications). The insert: Higher magnification (× 400) of the same section showing complete labelling of myocytes nuclei. () Myocytic necrosis visualized by the binding of antibody against the terminal complement complex (purple) to the sarcolemma. The longitudinal is from the left ventricle in a case of LLT group (× 200 magnification).Insert: Higher magnification (× 400) of the same section showing immunoreactivity of cardiac myocytes to the C9-mab. The endothelium of intramyocardial vessels also reacted with C9-ab and used as an internal control (arrow).<p><b>Copyright information:</b></p><p>Taken from "Pathogenic role of cardiac mast cell activation/degranulation, TNF-α, and cell death in acute drug-related fatalities"</p><p></p><p>Vascular Health and Risk Management 2007;3(6):1053-1062.</p><p>Published online Jan 2007</p><p>PMCID:PMC2350147.</p><p>© 2007 Perskvist et al, publisher and licensee Dove Medical Press Ltd.</p

Antibody responses after immunization with allergens.

<p>(A) quantification of IgE-responses in mice (n = 6) immunized with each rCan f 1, rCan f 2, rCan f 4, rCan f 6, an equimolar mix or Can f 1-2-4-6 (x-axis in graphs) to plates coated with anti-mouse IgE, mouse antiserum and biotinylated allergen (graph heading) by sandwich ELISA. (B) Comparison of IgG1 and IgG2a-antibodies to each lipocalin induced by solid phase Can f 1-2-4-6 or a mix. Immunoglobulin responses are presented as OD405 nm, y-axis. Boxes with median values and horizontal bars denote 50% of values and 1 standard deviation respectively. * p<0.05, ** p<0.01, *** p<0.001, analyzed with Kruskal-Wallis with Dunn's multiple comparison test (A, B, C) and Mann Whitney test (D).</p

Can f 1-2-4-6 structure based on solution small angle X-ray scattering data.

<p>(A) Experimental SAXS data from Can f 1-2-4-6 in solution (blue dots with error bars), computed fits from the CORAL (red line) and the GASBOR (grey line) models. (B) Respective pair distance distribution function. Maxima marked by vertical lines correspond to distances between the individual domains. (C) The rigid body model of Can f 1-2-4-6 reconstructed by the program CORAL resembles beads on a thread with each lipocalin forming a bead (cartoon representation) interspaced by extended linkers (light blue spheres). This model is in a good agreement with the <i>ab initio</i> GASBOR model (dummy residues represented by grey spheres).</p

Biochemical analysis of the linked molecule.

<p>(A) SDS-PAGE of Can f 1-2-4-6 or the single recombinant lipocalins visualized by coomassie staining under reducing conditions. MW markers (lane 1,7, MW (kDa)), rCan f 1 (lane 2), rCan f 2 (lane 3), rCan f 4 (lane 4), rCan f 6 (lane 5) and Can f 1-2-4-6 (lane 6). (B) Analytical size exclusion chromatography of rCan f 1-2-4-6 and the single lipocalin allergens. Molecular weight markers, bovine serum albumin (67 kDa), ovalbumin (43 kDa), chymotrypsinogen A (25 kDa) and Ribonuclease A (13.7 kDa). (C) Far-UV CD analysis of Can f 1-2-4-6 or an equimolar mix of the single recombinant allergens Can f 1, Can f 2, Can f 4 and Can f 6. The spectra are expressed as mean residue ellipticities (θ) at a given wavelength.</p