Injection intracytoplasmique de spermatozoide (analyse morphologique des échecs de fécondation)

PARIS5-BU Méd.Cochin (751142101) / SudocPARIS-BIUM (751062103) / SudocCentre Technique Livre Ens. Sup. (774682301) / SudocSudocFranceF

Genetic polymorphisms influence the ovarian response to rFSH stimulation in patients undergoing in vitro fertilization programs with ICSI.

INTRODUCTION: Obtaining an adequate number of high-quality oocytes is a major challenge in controlled ovarian hyperstimulation (COH). To date, a range of hormonal and clinical parameters have been used to optimize COH but none have significant predictive value. This variability could be due to the genetic predispositions of single-nucleotide polymorphisms (SNPs). Here, we assessed the individual and combined impacts of thirteen SNPs that reportedly influence the outcome of in vitro fertilisation (IVF) on the ovarian response to rFSH stimulation for patients undergoing intracytoplasmic sperm injection program (ICSI). RESULTS: Univariate analysis revealed that only FSHR, ESR2 and p53 SNPs influenced the number of mature oocytes. The association was statistically significant for FSHR (p=0.0047) and ESR2 (0.0017) in the overall study population and for FSHR (p=0.0009) and p53 (p=0.0048) in subgroup that was more homogeneous in terms of clinical variables. After Bonferroni correction and a multivariate analysis, only the differences for FSHR and ESR2 polymorphisms were still statistically significant. In a multilocus analysis, only the FSHR and AMH SNP combination significantly influenced oocyte numbers in both population (pA), ESR1(-397T>C), BMP15(-9C>G), MTHFR1(677C>T), MTHFR2(1298A>C), HLA-G(-725C>G), VEGF(+405G>C), TNFα(-308A>G), AMHR(-482 A>G), PAI-1 (4 G/5 G), multiplex PCR assay was designed to genotype women undergoing ICSI program. We analyzed the overall study population (n=427) and a subgroup with homogeneous characteristics (n=112)

Gene polymorphisms reportedly involved in IVF outcomes.

<p>Gene polymorphisms reportedly involved in IVF outcomes.</p

Electrophoregram profile with the 13 polymorphisms genotyped.

<p>The first and second lines show alleles fluorescently tagged with FAM and Hex dyes, respectively. The last electropherogram is the RO×500 size standard.</p

The p53 (Arg⁷²Pro) polymorphism: baseline population characteristics, treatment parameters and ovarian response.

<p>The major allele was used as a reference.</p>a,b,c<p>Univariate p value=0.0048, Univariate p after Holm’s correction=NS, multivariate p value=NS.</p>d<p>p value=0.0451.</p

Primer design for the selected SNPs.

<p>Primer design for the selected SNPs.</p

Allele frequencies of the studied SNPs in our population and in the NCBI database.

<p>Allele frequencies of the studied SNPs in our population and in the NCBI database.</p