MGL recognises CD45RA on human Tregs and modulates their suppressive capacity.

<p>(A) Flow cytometry analysis of purified Treg stained with anti- CD4, CD25, FOXP3 and CD45RA antibodies. (B) Competition studies of rhMGL-Fc binding to CD45RA expressed by Tregs using GalNAc polymer. Filled histograms represent the untreated Tregs, while the open histograms show Tregs treated with rhMGL-Fc or rhMGL-Fc+GalNAc. (C) Western blot analysis of Treg lysate immunoprecipitated with rhMGL-Fc. Samples were run in 4–12% SDS-PAGE gel and were analysed with anti-CD45RA and rhMGL-Fc. These results are representative of one donor out of three. (D) Immunosuppression capacity of Tregs alone and Tregs treated with rhMGL-Fc after four days of co-culture with CD4⁺ CD25^- T cells (ratio 1:5, Treg:CD4⁺CD25^- T cells), in presence of anti-CD3 and anti-CD28. (E) IFNγ spots produced by CD8+ T cells (5×10⁴/well) stimulated with mDCs in presence or not of Tregs ± rhMGL. The results correspond with the mean of three independent experiments ± standard deviation (SD). * corresponds to <i>p</i><0.05 and ** to p<0.01.</p