3 research outputs found
Bacterial translocation induces proinflammatory responses and is associated with early death in experimental severe injury
Objective: An experimental model of severe injury with great lethality
was studied to define the impact of bacterial translocation on survival
and on inflammatory response.
Methods: Forty-one rabbits were divided into two groups: A, femur
myotomy; and B, myotomy and fracture of the femoral bone. Vital signs
and survival were recorded. Serum circulating endotoxins
(lipopolysaccharides; LPS) were determined and tissue cultures were
performed at necropsy. A subgroup of animals was sacrificed at 48 h post
injury; LPS was determined in abdominal aorta and portal vein, apoptosis
of spleen cells was assessed by flow cytometry, and ex vivo production
of tumor necrosis factor alpha by splenocytes was measured.
Results: Tissue bacterial burden was increased in animals that died
early (i.e., within 48 h after injury) versus rabbits that died later.
Portal vein LPS at 48 h was increased in group B compared with group A,
whereas circulating LPS did not differ. No difference in apoptosis of
either lymphocytes or macrophages of the spleen was found in group B
compared with group A. Following stimulation with LPS or
phytohemagglutinin, tumor necrosis factor a production by splenocytes of
group B was greater than that of group A.
Conclusions: Bacterial translocation primes enhanced proinflammatory
responses and it is associated with early death in severe trauma. (C)
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