Histological aspects of the skin from different groups and subgroups.

<p><b>A. </b><b><i>Group 1</i></b>, UV non-protected regions 1. Intracellular edema and scattered inflammatory cells. HE, bar = 100 µm; <b>B</b> and <b>C. </b><b><i>Group 2</i></b>, UV non-protected regions; intracellular edema, perivascular edema and mononuclear inflammatory cells, dermal fibrosis. HE, bar = 100 µm; <b>D</b> and <b>E. </b><b><i>Group 3</i></b><b>,</b> UV non-protected regions. Intracellular edema, dermal fibrosis and mineralization of collagen fibers; HE, bar = 200 µm; multiple foci of epidermal necrosis. HE, bar = 100 µm; <b>F</b> and <b>G. </b><b><i>Group 4</i></b>, UV non-protected regions. F - Diffuse intracellular edema of epidermis and multiple apoptotic cells. HE, bar = 100 µm; G - Dermal fibrosis and perivascular mononuclear cell aggregation. HE, bar = 100 µm; <b>H. </b><b><i>Group 4</i></b><b>,</b> UV protected regions. Hyperkeratosis, epidermal atrophy and uniform dermal fibrosis. HE, bar = 100 µm.</p

The histological score of skin lesions on different age groups.

*<p>UV+ (UV nonprotected skin); **UV– (UV protected skin); ^#G0–G3 (Degree of skin lesions).</p

The percentage of immunostained fibroblasts and endothelial cells.

<p>SDV = Standard deviation.</p

Semiquantification of immunohistochemical CML staining.

*<p>UV+ (UV nonprotected skin); **UV – (UV protected skin); − nonreactive, + weak, ++ moderate, +++ strong intensity of CML.</p

Immunostaining for AGE-CML from UV non-protected areas: group 1(A), group 2 (C), group 3 (E), group 4 (G) and UV protected areas: group 1(B), group 2 (D), group 3 (F), group 4 (H).

<p>The presence of AGE-CML expression on fibroblasts, endothelial cells, epidermis and inflammatory cells. Haematoxylin counterstained (bar = 100 µm).</p

The graphical representation of the correlation analysis between the age of the patients and the AGE-CML score.

<p>(<b>A</b>) AGE-CML percentage is proportional with the patients’ age in endothelial cells in protected regions (Pearson correlation coefficient = 0.941). (<b>B</b>) AGE-CML percentage is proportional with the patients’ age in fibroblasts in protected regions (Pearson correlation coefficient = 0.921). (<b>C</b>) AGE-CML percentage is proportional with the patients’ age in endothelial cells in non-protected regions (Pearson correlation coefficient = 0.912). (<b>D</b>) AGE-CML percentage is proportional with the patients’ age in fibroblasts in non-protected regions (Pearson correlation coefficient = 0.949).</p

AGE-CML expression in fibroblasts and endothelial cells from both UV protected and non-protected sites.

<p>(<b>A</b>) AGE-CML is statistically higher in non-protected fibroblasts than in protected tissues (paired t-test probability is less than 0.001); (<b>B</b>) AGE-CML is statistically higher in non-protected endothelial cells than in protected tissues (paired t-test probability is less than 0.001).</p