S100A7 (Psoriasin), highly expressed in Ductal Carcinoma (DCIS), is regulated by IFN-gamma in mammary epithelial cells-1

<p><b>Copyright information:</b></p><p>Taken from "S100A7 (Psoriasin), highly expressed in Ductal Carcinoma (DCIS), is regulated by IFN-gamma in mammary epithelial cells"</p><p>http://www.biomedcentral.com/1471-2407/7/205</p><p>BMC Cancer 2007;7():205-205.</p><p>Published online 6 Nov 2007</p><p>PMCID:PMC2180183.</p><p></p> reaction (RT-PCR), mRNA level of psoriasin was downregulated in response to IFN-gamma in MCF10A cells () and in MDA-MB-468 cells (). Expression data for psoriasin are presented as ratios, in which the expression data are normalized to an endogenous control (β-actin). Values obtained in control cells were designed as 1 and values obtained from IFN-gamma treated cells were normalized to this from the same run. The data are presented as the mean of three different runs

S100A7 (Psoriasin), highly expressed in Ductal Carcinoma (DCIS), is regulated by IFN-gamma in mammary epithelial cells-3

<p><b>Copyright information:</b></p><p>Taken from "S100A7 (Psoriasin), highly expressed in Ductal Carcinoma (DCIS), is regulated by IFN-gamma in mammary epithelial cells"</p><p>http://www.biomedcentral.com/1471-2407/7/205</p><p>BMC Cancer 2007;7():205-205.</p><p>Published online 6 Nov 2007</p><p>PMCID:PMC2180183.</p><p></p>MTS-assay on days 3 and 7 and the IFN-gamma/control quotient is demonstrated. Treatment with IFN-gamma produced a 59.5% (day 3) and 32% (day 7) reduction in viability as compared to control cells. The assay was performed in duplicate

S100A7 (Psoriasin), highly expressed in Ductal Carcinoma (DCIS), is regulated by IFN-gamma in mammary epithelial cells-6

<p><b>Copyright information:</b></p><p>Taken from "S100A7 (Psoriasin), highly expressed in Ductal Carcinoma (DCIS), is regulated by IFN-gamma in mammary epithelial cells"</p><p>http://www.biomedcentral.com/1471-2407/7/205</p><p>BMC Cancer 2007;7():205-205.</p><p>Published online 6 Nov 2007</p><p>PMCID:PMC2180183.</p><p></p>00 u or 1000 u of IFN-gamma. IFN-gamma treatment had no effect on the protein levels of pro-caspase-3. Calgranulin B and calgranulin A () are not influenced by the IFN-gamma treatment. Equal amounts of protein lysate were loaded on the gel. , Treatment with 75 μM HOfor one hour followed by culture for 48 h in regular medium induces the expression of calgranulin A in MCF10A cells. 100 μg of protein lysate were loaded on the gel. , MDA-MB-468 cells with the endogenous expression of psoriasin show the time-dependent repression of psoriasin expression when treated 24, 48 and 72 hours with 1000 u of IFN-gamma. Equal amounts of protein lysate were loaded on the gel. Probing with tubulin/GAPDH assesses the equal loading of the samples. C = cells cultured in monolayer

S100A7 (Psoriasin), highly expressed in Ductal Carcinoma (DCIS), is regulated by IFN-gamma in mammary epithelial cells-4

<p><b>Copyright information:</b></p><p>Taken from "S100A7 (Psoriasin), highly expressed in Ductal Carcinoma (DCIS), is regulated by IFN-gamma in mammary epithelial cells"</p><p>http://www.biomedcentral.com/1471-2407/7/205</p><p>BMC Cancer 2007;7():205-205.</p><p>Published online 6 Nov 2007</p><p>PMCID:PMC2180183.</p><p></p>on with dnIkkβ-virus in MCF10A cells. Neither infection with the different adenoviruses nor the IFN-gamma had any effect on the protein levels of pro-caspase-3. The function of the virus was verified by the accumulation of the IκBα protein. Tubulin assesses equal loading. C control