Patient disposition flow diagram.

<p>Patient disposition flow diagram showing patients included, excluded or lost-to-follow-up within the study cohort. MS: Multiple Sclerosis; RRMS: Relapsing-Remitting Multiple Sclerosis.</p

Additional file 1: Table S1. of Risk factors for Candida parapsilosis bloodstream infection in a neonatal intensive care unit: a case-control study

PCR fingerprinting profiles of C. parapsilosis isolates. Table S2. Robust ANOVA and Kruskal-Wallis tests. (PDF 71 kb

Healthcare costs for MS treatment and the rate of relapse occurrence, of 1-point EDSS progression, of reaching of EDSS 4.0, of reaching of EDSS 6.0, and of SP conversion.

<p>Healthcare costs for DMT administration and management before the specific endpoint was reached (considered as continuous variables), have been associated with the rate of relapse occurrence, of 1-point disability progression, of reaching of EDSS 4.0, of reaching of EDSS 6.0, and of SP conversion during the follow-up period. P-values, hazard ratios (HR), and 95% confidence intervals (95%CI) are shown from time varying Cox regression models, subsequently adjusted for age, gender, disease duration, and baseline EDSS.</p

Kaplan-Meier curves for the probability of relapse occurrence, of 1-point EDSS progression, of reaching of EDSS 4.0, of reaching of EDSS 6.0, and of SP conversion, in relation to annual healthcare costs before the specific study endpoint was reached.

<p>Kaplan-Meier plots estimating the probability of relapse occurrence (<b>A</b>), of experiencing 1-point EDSS progression (<b>B</b>), of reaching of EDSS 4.0 (<b>C</b>), of reaching of EDSS 6.0 (<b>D</b>), and of SP conversion (<b>E</b>), in relation to the annual healthcare costs before the specific study endpoint was reached. P-values and hazard ratios (HR) are shown from time varying Cox regression models. For graphical purposes, healthcare costs have been categorized on the median value (the red line represents costs lower than the median value, whereas the blue line represents costs higher than the median value). EDSS: Expanded Disability Status Scale; HR: Hazard Ratio.</p

Scatter plot for overall annual healthcare costs and relapses.

<p>Scatter plot showing the relationship between overall annual healthcare costs and the ARR. P-value and coefficient from Poisson regression analysis are shown; 95% confidence intervals are represented in grey shadow. ARR: Annualised Relapse Rate; Coef: Coefficient.</p

Demographic features, clinical findings and healthcare costs.

<p>Demographic features, clinical findings and annual healthcare costs for DMT administration and management.</p

Correlation between the clinical variables and tumor-associated CD66b⁺ neutrophils in thyroid carcinoma.

<p>Correlation between the clinical variables and tumor-associated CD66b⁺ neutrophils in thyroid carcinoma.</p

The number of tumor-infiltrating neutrophils positively correlated with tumor size in human TC specimens.

<p><b>A and B.</b> Histological analysis of TC specimens stained with a monoclonal anti-CD66b antibody. Whole-tumor section density of CD66b⁺ neutrophils was scored at 200× magnification. Representative cases of papillary thyroid carcinomas with a high <b>(A)</b> and low <b>(B)</b> CD66b⁺ neutrophil count (arrows; hematoxylin counterstaining, 200×). <b>C.</b> CD66b⁺ neutrophil counts in tumor specimens were distributed according to the tumor size. The median value of tumor size served as a cutoff level. Results are shown as the median, the 25th and 75th percentiles (boxes), and 5th and 95th percentiles (whiskers); *p < 0.05, according to the two-tailed Mann–Whitney <i>U</i> test. <b>D.</b> Neutrophil density positively correlated with larger tumor size in TC patients (r = 0.43; p = 0.01; Pearson’s correlation test).</p

TC-derived soluble factors modified neutrophils’ kinetic properties.

<p>Neutrophils were stimulated with a TC-CM or the control medium for 18 hours. Within this time window, digital phase contrast images of 15 fields/well were captured every 15 minutes via a 20× objective in the Operetta high-content imaging system. PhenoLOGIC (PerkinElmer) was employed for image segmentation and to calculate the single-cell kinetic properties <b>(A)</b>, accumulated distance, <b>(B)</b> speed and straightness <b>(C)</b> in dedicated analysis sequence. The results were expressed as mean ± SEM of six independent experiments; ***p < 0.005; **p < 0.01; *p < 0.05. <b>D-E.</b> Timepoint analyses of time-dependent properties such as current step size (D) and current speed (E) illustrating dynamic changes of the behavior of neutrophils (in presence or absence of TC-CMs) in function of the elapsed time from treatment. The results were expressed as mean ± SEM of six independent experiments; ****p < 0.001.</p

TC-derived soluble factors promoted neutrophil survival.

<p><b>A.</b> Neutrophils were cultured in a TC-CM or the control medium. At the indicated time points, live cells were evaluated by flow cytometry with FITC-conjugated annexin V and PI. Results were expressed as percentages of live cells (mean ± SEM of five independent experiments); ***p < 0.005; **p < 0.01; *p < 0.05. <b>B.</b> Representative flow cytometric panels of dot plots of PMNs cultured in a TC-CM or control medium and stained with FITC-conjugated annexin V and propidium iodide (PI) at 24 (upper panels) and 48 (lower panels) hours. <b>C</b>. The GM-CSF release by TPC1 and 8505c cells was evaluated by an ELISA in a TC-CM or in the control medium. Results were expressed as mean ± SEM of seven independent experiments; ****p < 0.001; ***p < 0.005. <b>D-F.</b> Neutrophil survival in a TPC1-derived <b>(D-E)</b> or 8505c-derived <b>(F-G)</b> conditioned medium was evaluated in the presence of an anti-GM-CSF blocking antibody or the relative isotype control (10 μg/ml). At 24 hours, live cells were stained with FITC-conjugated annexin V and PI and analyzed by flow cytometry. <b>Figs E and G</b> illustrate representative flow cytometric panels of one out of five independent experiments. The results were expressed as mean ± SEM of five independent experiments; **p < 0.01; *p < 0.05.</p