Characteristics and comparisson of the population from Group 1, according to their GBV-C status at baseline, N = 233.

<p>*Pearson's chi-squared test.</p><p>**Wilcoxon rank-sum test adjusted for ties.</p

Phylogenetic tree of GB virus C (GBV-C) isolates.

<p>Phylogenetic tree of Brazilian GB virus C (GBV-C) clinical isolates based on the nucleotide sequences. Tree was constructed by the Neighbor-Joining (NJ) program, and the pair wise distances between sequences were calculated by the DNADIST program. Values near branches indicate bootstrap support based on 1000 replicates. Relevant bootstrap values >60 (out of 100) are shown.</p

Characteristics of the GBV-C positive population, according to their genotypic distribution, N = 45.

<p>*Two-sided Fisher's exact test.</p><p>**Kruskal-Wallis test (chi-square approximation).</p

Characteristics of the population from Group 2, according to their E2 Antibody status at baseline, N = 131.

<p>*Pearson's chi-squared test.</p><p>**Kruskal-Wallis test (chi-square approximation) adjusted for tiés.</p

Associations between inferred tropism and time to CD4 decline to levels below 350 cells/µL (survival probability).

<p>There was a statistically significant association between the presence of CXCR4-using viruses (X4) in the genotype at baseline and an earlier time to CD4 decline below 350 cells/µL (log rank P = 0.0450). The mean time to CD4 decline below 350 was 51.31±3.66 and 34.14±10.61 months for R5- and X4-infected individuals, respectively.</p

CD4 decline over time in individuals infected with R5 or CXCR4-using viruses (mean initial CD4+ T cell counts of 558.5 and 481.4 respectively).

<p>CD4 decline over time in individuals infected with R5 or CXCR4-using viruses (mean initial CD4+ T cell counts of 558.5 and 481.4 respectively).</p

Characteristics of individuals infected by different tropic HIV.

<p>*Men who have sex with men.</p><p>At the tip of V3 loop, strains were classified as B if they harbor a proline or B″ if they harbor a tryptophan or a related amino acid.</p

Phylogenetic relationships between analyzed samples.

<p>D85_40 at the top is the North-American/European B consensus sequence. * indicates CXCR4-using strains. Bootstrap values above 85% are indicated at the intersections of branches.</p

Amino acid alignment of V3 sequences.

<p>Subtype B sequences were aligned with the consensus sequence. Amino acid positions appear at the top of each alignment, with dots indicating identities whereas dashes indicate deletions. # indicates the N-linked glycosylation site, * indicates the GPGR motif at the tip of the V3 loop, and § indicates positions 316 and 323 (HXB2 positions), which are associated with in vitro selection of maraviroc resistance (316T and 323 V substitutions are shaded) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030292#pone.0030292-Westby1" target="_blank">[29]</a>. Inferred tropism is indicated to the right of each sequence (CXCR4-using strains are named X4 for the sake of simplicity).</p