Progress of targeted acquisition versus total measurement time for a 120 µM sample of CD79a.

<p>Build-ups are shown for the number of assigned residues and the number of detected peaks in individual BEST-TROSY-type experiments <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062947#pone.0062947-Favier1" target="_blank">[14]</a>.</p

Zoomed region of the ¹⁵N-HSQC of CD79a in different conditions.

<p>(<b>A</b>) NaPi buffer, (<b>B</b>) 6 M urea, (<b>C</b>) 20% TFE, (<b>D</b>) reduced spin label (MTSL) attached to CD79a, (<b>E</b>) K4C/C35S form, (<b>F</b>) Y25E/Y36E form. Selected peaks are annotated to show rearrangements of the signal position for the different conditions (19Y, 35M) or position of the specific mutations (4K, 33C, 25Y, 36Y). Peaks outside the zoomed region are shown as arrows pointing towards the correct position.</p

Cell-free expressed cytosolic constructs of the T cell- and B cell receptor.

<p>(<b>A</b>) Cartoon of the receptors with indicated immunoreceptor tyrosine-based activation motifs (ITAMs), (<b>B</b>) SDS-PAGE gel of <i>in vitro</i> expressed disordered constructs in levels suitable for NMR experiments.</p

Assignment validation procedure with jackknife resampling.

<p>See text for explanations.</p