Analysis of the envelope (E) gene sequence of SLEV.

<p>Phylogenetic tree was constructed using partial E gene sequence by neighbor-joining (NJ) method (using p-distance) of 1,000 bootstrap replicates using Mega 4.0.2. Boostraps values above 60% are shown. The scale bar represents 5% nucleotide sequence divergence. WNV, JEV and DENV-1 were used as the out group.</p

Central nervous system from newborn mice infected with SLEV.

<p>(A) Cerebral cortex from a newborn mouse at the 4^th viral passage with mild hyperemia and mild lympho-histiocytic perivascular cuffs, HE, 600×. (B) Choroid plexus from a newborn mouse at the 4^th viral passage, focally extensive hemorrhage in the ventricular cavity, HE, 200×. (C) Heart from a newborn at the 5^th viral passage, with moderate hyperemia and mild multifocal hemorrhage in the myocardium, HE, 200×. (D) Liver from a newborn at the 6^th viral passage, with severe hyperemia and focal hemorrhage, HE, 200×. (E) Lung from a newborn at the 7^th viral passage, with severe hyperemia, HE, 200×.</p

Viral strains included in phylogenetic analysis.

*<p>Designation according to country or state (in the case of USA) and year of virus isolation. Abbreviations: CA, California; TX, Texas; NM, New Mexico; TN, Tennessee; FL, Florida; MD, Maryland; PAN, Panama; MEX, Mexico; GUA, Guatemala; ARG, Argentina; BRA, Brazil; WNV, West Nile Virus; JEV, Japanese Encephalitis Virus; and DENV, Dengue Virus.</p

Survival rate and frequency of clinical signs in newborn mice inoculated with SLEV.

<p>Uninfected control mice (control), first (P1), second (P2), Third (P3), 4^th (P4), 5^th (P5), 6^th (P6), and 7^th (P7) passages are indicated in the X axis.</p

Newborn mice inoculated with SLEV presenting neurological and circulatory disorders at the 3^rd viral passage.

<p>(A) Bending of the spine (thoracic kyphosis), and limited mobility. (B) Front limb flexed characterizing loss of proprioception and apathy. (C) Necrosis of the distal extremity of the hind limbs and at the tip of the tail. (D) Hyperemia and extensive areas of hemorrhage in the meninges at the rostral part of the brain.</p