2 research outputs found
Development of Clickable Monophosphoryl Lipid A Derivatives toward Semisynthetic Conjugates with Tumor-Associated Carbohydrate Antigens
A semisynthetic strategy to obtain
monophosphoryl lipid A derivatives
equipped with clickable (azide, alkyne, double bond, or thiol precursor)
moieties, starting from the native lipid A isolated from <i>Escherichia
coli</i>, is presented. These lipid A derivatives can be conjugated
with other interesting biomolecules, such as tumor-associated carbohydrate
antigens (TACAs). In this way, the immunostimulant activity of monophosphoryl
lipid A can significantly improve the immunogenicity of TACAs, thus
opening access to potential self-adjuvant anticancer vaccine candidates.
A monophosphoryl lipid A–Thomson-Friedenreich (TF) antigen
conjugate was obtained to demonstrate the feasibility of this methodology,
which stands as a valuable, rapid, and scalable alternative to the
highly complex approaches of total synthesis recently reported to
the same aim. A preliminary evaluation of the immunological activity
of this conjugate as well as of other semisynthetic lipid A derivatives
was also reported
Inside out <i>Porphyridium cruentum</i>: Beyond the Conventional Biorefinery Concept
Here, an unprecedented
biorefinery approach has been designed to
recover high-added value bioproducts starting from the culture ofPorphyridium cruentum. This unicellular marine red
alga can secrete and accumulate high-value compounds that can find
applications in a wide variety of industrial fields. 300 ± 67
mg/L of exopolysaccharides were obtained from cell culture medium;
phycoerythrin was efficiently extracted (40% of total extract) and
isolated by single chromatography, with a purity grade that allowed
the crystal structure determination at 1.60 Å; a twofold increase
in β-carotene yield was obtained from the residual biomass;
the final residual biomass was found to be enriched in saturated fatty
acids. Thus, for the first time, a complete exploitation ofP. cruentumculture was set up