13 research outputs found

    Elements of oxidation/reduction balance in experimental hypothyroidism

    Get PDF
    Background: The aim of this study was to investigate the effect of the decreased level of thyroid hormones on selected parameters of the oxidation/reduction balance by assessing the activity of antioxidant enzymes: superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px); the level of antioxidant vitamins (A, C, and E); and the concentration of compounds reacting with thiobarbituric acid (TBARS). Material and methods: Investigations involved 20 Belgian giant rabbits of both sexes. Hypothyroidism was induced by intragastric administration of thiamizole. Before this was done, blood was collected from the ear marginal vein (control group) and then the animals received thiamizole through an intragastric tube at a dose of 2 mg/kg b.w. for 21 days. Blood was collected again (the experimental group) and the following determinations were performed: — in blood serum, the thyroid hormones T3, T4 and TSH; — vitamin A, C and E blood serum concentrations; — in erythrocytes, the concentration of compounds reacting with TBARS, SOD and GSH-Px. Results: A 21-day exposure of rabbits to thiamazole (2 mg/kg b.w./24 h) resulted in a statistically significant decrease of TBARS, a decrease of SOD and GPH-Px activity and in a statistically insignificant decrease in the level of vitamins A, C and E. Conclusions: Hypothyroidism decreases the level of erythrocytes oxidation/reduction balance by diminishing oxidative lipids damage and by decreasing the activity of antioxidative enzymes, but not by changes in the level of antioxidant vitamins. (Pol J Endocrinol 2011; 62 (3): 220–223)Wst臋p: Celem badania by艂a ocena wp艂ywu zmniejszenia st臋偶enia hormon贸w tarczycy na wybrane parametry r贸wnowagi oksydacyjno-redukcyjnej przez badanie aktywno艣ci enzym贸w antyoksydacyjnych ( SOD, GSH-Px), st臋偶enia witamin antyoksydacyjnych (A, C, E) oraz zwi膮zk贸w reaguj膮cych z kwasem tiobarbiturowym (TBARS). Materia艂 i metody: Badania przeprowadzono na 40 kr贸likach rasy olbrzym beligijski, obojga p艂ci. Niedoczynno艣膰 tarczycy wywo艂ano do偶o艂膮dkowym podawaniem thiamazolu. Przed podaniem thiamazolu pobrano krew 偶yln膮 (grupa kontrolna), a nast臋pnie przez 21 dni zwierz臋ta otrzymywa艂y do偶o艂膮dkowo thiamazol w dawce 2 mg/kg mc. Po tym czasie ponownie pobierano krew (grupa badana) i oznaczano: — st臋偶enie hormon贸w tarczycy T3, T4 i TSH; — st臋偶enie witamin A, C i E w surowicy; — st臋偶enie zwi膮zk贸w reaguj膮cych z kwasem tiobarbiturowym (TBARS) i aktywno艣膰 dysmutazy ponadtlenkowej (SOD) i peroksydazy glutationowej (GPH-Px) w erytrocytach. Wyniki: Po 21 dniach do偶o艂膮dkowego podawania kr贸likom thiamazolu (2 mg/kg mc./24 h) stwierdzono statystycznie znamienne zmniejszenie st臋偶enia TBARS, obni偶enie aktywno艣ci SOD i GPH-Px oraz statystycznie nieznamienne obni偶enie st臋偶enia witaminy A, C i E. Wnioski: Niedoczynno艣膰 tarczycy obni偶a poziom r贸wnowagi oksydacyjno-redukcyjnej erytrocyt贸w poprzez zmniejszenie oksydacyjnych uszkodze艅 t艂uszcz贸w oraz zmniejszenie aktywno艣ci enzym贸w antyoksydacyjnych, a nie poprzez zmiany w st臋偶eniu witamin antyoksydacyjnych. (Endokrynol Pol 2011; 62 (3): 220–223

    Effect of anthocyanins on selected biochemical parameters in rats exposed to cadmium.

    No full text
    Cadmium is a dangerous occupational and environmental toxin. It accumulates in the human organism mainly in liver and kidneys. Cadmium half-life is about 10 years, so the symptoms of cadmium intoxication may occur several years after the exposure. Until now in treating intoxication with this metal chelating compounds have been used, burdened with numerous undesirable symptoms. In our investigations anthocyanins from Aronia melanocarpa were used to reduce the harmful results caused by cadmium. Administering anthocyanins with cadmium chloride resulted in a statistically significant decrease of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity, concentration of bilirubin and urea in blood serum and decreased cadmium cumulation in liver and kidneys in relation to animals receiving cadmium chloride only

    Prace oryginalne/original PaPers Elements of oxidation/reduction balance in experimental hypothyroidism Elementy bariery oksydacyjno-redukcyjnej w do艣wiadczalnej hypotyreozie

    No full text
    Abstract Background: The aim of this study was to investigate the effect of the decreased level of thyroid hormones on selected parameters of the oxidation/reduction balance by assessing the activity of antioxidant enzymes: superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px); the level of antioxidant vitamins (A, C, and E); and the concentration of compounds reacting with thiobarbituric acid (TBARS). Material and methods: Investigations involved 20 Belgian giant rabbits of both sexes. Hypothyroidism was induced by intragastric administration of thiamizole. Before this was done, blood was collected from the ear marginal vein (control group) and then the animals received thiamizole through an intragastric tube at a dose of 2 mg/kg b.w. for 21 days. Blood was collected again (the experimental group) and the following determinations were performed: -in blood serum, the thyroid hormones T3, T4 and TSH; -vitamin A, C and E blood serum concentrations; -in erythrocytes, the concentration of compounds reacting with TBARS, SOD and GSH-Px
    corecore