1 research outputs found
Enhanced in Vitro Biological Activity of Synthetic 2β(2-Pyridyl) Ethyl Isothiocyanate Compared to Natural 4β(Methylsulfinyl) Butyl Isothiocyanate
Dietary isothiocyanates (ITC) derived from cruciferous
vegetables
have been shown to have numerous biological effects consistent with
chemoprotective activity. In this study we synthesized a novel ITC,
2-(2-pyridyl) ethyl ITC (PY-ITC), and assessed its chemopreventive
ability in comparison to sulforaphane (SF), the ITC derived from broccoli.
PY-ITC suppressed cancerous cell growth and proliferation at lower
concentrations than SF and was more potent at inducing p21 protein.
Through the use of whole genome arrays we demonstrate that prostate
cells exposed to PY-ITC or SF have similar biological response, albeit
PY-ITC alters a greater number of genes, and to a greater extent.
In the presence of a phosphatidylinositol-3-kinase (PI3K) inhibitor
PY-ITC had a more pronounced effect on gene expression, emphasizing
the important role of PI3K/AKT signaling in mediating the chemopreventive
effects of ITCs. These results highlight the importance of the ITC
side chain in bioactivity