Development of a PCR assay for the detection of animal tissues in ruminant feeds.

The European Community ban on use of meat and bone meal in ruminant feed, as a consequence of the spread of bovine spongiform encephalopathy in Europe, has prompted a number of investigations about the possibility of detecting animal tissues in feedstuff. In this paper, a study on vertebrate primers, designed in the 16S rRNA gene of mitochondrial DNA, is described. These primers were able to amplify fragments that contained between 234 and 265 bp. The fragments were specific for bovine, porcine, goat, sheep, horse, rabbit, chicken, trout, and European pilchard and were confirmed by sequence analysis amplicons. The primers were used in a PCR assay applied to five samples of meat and blood meals of different species and subjected to severe rendering treatments (134.4 to 141.9 degrees C and 3.03 to 4.03 bar for 24 min). The presence of vertebrate tissues was detected in all samples. The assay proved to be rapid and sensitive (detection limit 0.0625%). It can be used as a routine method to detect animal-derived ingredients in animal feedstuff

Travels of a Rayed Head: imagery, fiber, structure and connotations of early textiles from the South Central Andes

The rayed head image has long been identified as a central symbol associated with the Paracas tradition, also called the sun face 1 and associated with the concept of Oculate Being developed by the Berkeley School. 2 Prominently repeated on the central ground of the famous Paracas Textile at the Brooklyn Museum, this image has much earlier antecedents in the region. Scholars disagree on the extent to which many Paracas, Topara and early Nasca images with large round eyes, grinning mouths, and serpentlike appendages emitting from the head and body may also be manifestations of a particular Oculate Being or of more general concepts of natural or supernatural power. Recently, contemporary textiles found in the Sihuas valley to the south (see Haeberli in this volume) challenge us to reexamine the similarities and distinctions among rayed heads. One of the great challenges of the history of material culture, envisioned as a history of philosophical concepts, social values and cultural practices through their inscription in material objects, is the degree to which a recurrent image, pattern or special arrangement reflects a similar idea. A number of quite different images have been associated with the concept of an Oculate Being proposed by John Rowe and others of the Berkeley school based on their analysis of Ica valley ceramics and Ocucaje gravelots in the 1950s. I here trace the rayed head or sun face image as it occurs over at least 500 years in the region of Ica and Paracas. I then briefly consider its relationship to other contemporary imagery and later imagery featuring ray-like elements emitted from the head, both in the same contexts where the rayed head appears, and in other cemeteries to the south in southern Peru and northern Chile. All the imagery discussed here is associated with a period between about 450 BC and AD 450 called the Formative in the South Central Andes (Bolivia and northern Chile) and called the Early Horizon (or late Formative) and Early Intermediate (or Regional Development) Period in the Central Andes. Most of the images I discuss are created on textiles. While only recovered from burials on the desert coast, textile materials draw on relationships of production and exchange that spanned the Andean cordillera to the montane rainforest to the east, and stretched to the north and south. Either as clothing or cargo, textiles themselves traveled and were no doubt a primary source of non-local imagery. I do consider related images on non-textile artifacts. I compare textile based imagery with contemporary imagery on engraved and painted ceramics and gourds to try to distinguish among design features specific to medium, style and iconography

Anthropic Pressure on Cetaceans Stranded Along the Ligurian Coast Within the Pelagos Sanctuary: A Case Series

Simple Summary: In recent decades, environmental changes consequent to human activities have become more important factors in the conservation of wild species. Despite the Ligurian sea being part of the Pelagos Sanctuary, which has been a Specially Protected Areas of Mediterranean Importance (SPAMI) and a Marine Protected Area (MPA) since 1999, several anthropogenic threats affecting the health of cetacean populations living in this area are known to be present. In particular, interaction with fishing activities, contamination with chemical compounds such as organochlorine compounds (OCs), interaction with marine debris (especially, plastic debris), and the emergence of infectious agents, many of which are of terrestrial origin as a result of contamination of the marine environment by agricultural, animal, and human waste, are just some of the threats. In this regard, cetaceans stranded along the Ligurian coast between 2020 and 2022 were evaluated by means of a comprehensive post mortem examination and thorough specific diagnostic investigations to assess the level of anthropic pressure in this area by the Italian National Reference Center for Diagnosis of stranded Marine Mammals (C. Re. Di. Ma). Abstract: Data collected by C. Re. Di. Ma over a 3-year period (2020–2022) were considered to assess anthropic pressure on cetaceans living in the Ligurian sea. Out of a total of 37 stranded cetaceans, a complete post mortem examination was performed on 23 cases. Of these, 14 were further selected considering at least one of these conditions: (i) confirmed, probable, or suspected interaction with fishing activities through the application of a standardized diagnostic framework (7/14; 50%), (ii) toxicological stress through the evaluation of OCs hazardous levels (14/14; 100%), and (iii) terrestrial pathogen-associated disease (systemic infection and/or associated lesions) (7/14; 50%). For 9 animals out of a total of 14 selected, the cause of death was classified as natural (6/14; 42,8%), anthropic (3/14; 21,4%), or not determined (5/14; 35,7%) based on gross and histological pathology and ancillary testing. These findings extend our knowledge of the anthropic pressure to which cetaceans stranded along the Ligurian coastline are subjected from a multidisciplinary point of view

Application of Technological Control Measures on Vehicle Pollution: A Cost-Benefit Analysis in China

For the past two decades, China has experienced strong, continuous economic growth. At the same time, the number of motor vehicles in China has rapidly increased. As a direct result of such a phenomenon, China has been registering significant increases in air pollution. In spite of recent advances in air pollution control, it remains a serious problem for Chinas major cities, and constitutes an important issue in the agenda of its policy makers. The object of this paper is to explore the use of cost-benefit analysis (CBA) to evaluate and rank alternative policy scenarios regarding the control of air pollution emitted by motor vehicles. The empirical analysis carried out relates specifically to the Chinese context, over a twenty year period, from 2001 to 2020, and focuses on emission changes of the following three principal pollutants: CO, HC and NOx

Strategic Intellectual Property Rights Policy and North-South Technology Transfer

This paper analyzes welfare implications of protecting intellectual property rights (IPR) in the framework of TRIPS for developing countries (South) through its impact on innovation, market structure and technology transfer. In a North-South trade environment, the South sets its IPR policy strategically to manipulate multinationals decisions on innovation and location. Firms can protect their technology by exporting or risk spillovers by undertaking FDI to avoid tariffs. A stringent IPR regime is always optimal for the South as it triggers technology transfer by inducing FDI in less R&D-intensive industries and stimulates innovation by pushing multinationals to deter entry in high-technology sectors

Impure Public Goods and Technological Interdependencies

Impure public goods represent an important group of goods. Almost every public good exerts not only effects which are public to all but also effects which are private to the producer of this good. What is often omitted in the analysis of impure public goods is the fact that – regularly – these private effects can also be generated independently of the public good. In our analysis we focus on the effects alternative technologies – independently generating the private effects of the public good – may have on the provision of impure public goods. After the investigation in an analytical impure public good model, we numerically simulate the effects of alternative technologies in a parameterized model for climate policy in Germany

Precautionary Effect and Variations of the Value of Information

For a sequential, two-period decision problem with uncertainty and under broad conditions (non-finite sample set, endogenous risk, active learning and stochastic dynamics), a general sufficient condition is provided to compare the optimal initial decisions with or without information arrival in the second period. More generally the condition enables the comparison of optimal decisions related to different information structures. It also ties together and clarifies many conditions for the so-called irreversibility effect that are scattered in the environmental economics literature. A numerical illustration with an integrated assessment model of climate-change economics is provided

Bargaining with Non-Monolithic Players

This paper analyses strategic bargaining in negotiations between non-monolithic players, i.e. agents starting negotiations can split up in smaller entities during the bargaining process. We show that the possibility of scission in the informed coalition implies that it loses its information advantages. We also show that when the possibility of a scission exists the uninformed player does not focus on his or her beliefs about the strength of the informed coalition but on the proportion of weak/strong players within this coalition. Finally, our results show that the possibility of a scission reduces the incentives for the leader to propose a high offer to ensure a global agreement. We apply this framework to international negotiations on global public goods and to wage negotiations