Bootscan analysis of HIV-1 multiple infections based on the protease gene.

<p>We used the Neighbor-Joining method with K2P and 1000 bootstrap replicates. This analysis was performed using the software Simplot v3.5.1, with a window of 75 bp and 5 bp increments.</p

Schematic mosaic structures and pure subtypes found in multiple infections.

<p>The number of times that a particular subtype or recombinant was found in a patient is shown inside the parenthesis to the left of each structure. Break points were mapped according to Bootscan and their positions in base pairs are followed by the number they appeared on mosaic structures (inside the parenthesis).</p

Protease gene break point positions in nucleotides and their frequency.

<p>X axis indicates the number each breakpoint was detected in our study. Break points detected in single patients are represented by Y axis bars in blue whereas; break points detected in multiple patients appear in multicolored bars. The numbers inside parenthesis are the number of different patients in which this particular break point was detected.</p

Breakpoints and Recombination Individuals.

<p>Breakpoints and Recombination Individuals.</p

Characteristics of the GBV-C positive population, according to their genotypic distribution, N = 45.

<p>*Two-sided Fisher's exact test.</p><p>**Kruskal-Wallis test (chi-square approximation).</p

Characteristics of the population from Group 2, according to their E2 Antibody status at baseline, N = 131.

<p>*Pearson's chi-squared test.</p><p>**Kruskal-Wallis test (chi-square approximation) adjusted for tiés.</p

Phylogenetic tree of GB virus C (GBV-C) isolates.

<p>Phylogenetic tree of Brazilian GB virus C (GBV-C) clinical isolates based on the nucleotide sequences. Tree was constructed by the Neighbor-Joining (NJ) program, and the pair wise distances between sequences were calculated by the DNADIST program. Values near branches indicate bootstrap support based on 1000 replicates. Relevant bootstrap values >60 (out of 100) are shown.</p

Characteristics and comparisson of the population from Group 1, according to their GBV-C status at baseline, N = 233.

<p>*Pearson's chi-squared test.</p><p>**Wilcoxon rank-sum test adjusted for ties.</p