9 research outputs found
A framework for the targeted recruitment of crop-beneficial soil taxa based on network analysis of metagenomics data
Background The design of ecologically sustainable and plant-beneficial soil systems is a key goal in actively manipulating root-associated microbiomes. Community engineering efforts commonly seek to harness the potential of the indigenous microbiome through substrate-mediated recruitment of beneficial members. In most sustainable practices, microbial recruitment mechanisms rely on the application of complex organic mixtures where the resources/metabolites that act as direct stimulants of beneficial groups are not characterized. Outcomes of such indirect amendments are unpredictable regarding engineering the microbiome and achieving a plant-beneficial environment.Results This study applied network analysis of metagenomics data to explore amendment-derived transformations in the soil microbiome, which lead to the suppression of pathogens affecting apple root systems. Shotgun metagenomic analysis was conducted with data from 'sick' vs 'healthy/recovered' rhizosphere soil microbiomes. The data was then converted into community-level metabolic networks. Simulations examined the functional contribution of treatment-associated taxonomic groups and linked them with specific amendment-induced metabolites. This analysis enabled the selection of specific metabolites that were predicted to amplify or diminish the abundance of targeted microbes functional in the healthy soil system. Many of these predictions were corroborated by experimental evidence from the literature. The potential of two of these metabolites (dopamine and vitamin B-12) to either stimulate or suppress targeted microbial groups was evaluated in a follow-up set of soil microcosm experiments. The results corroborated the stimulant's potential (but not the suppressor) to act as a modulator of plant beneficial bacteria, paving the way for future development of knowledge-based (rather than trial and error) metabolic-defined amendments. Our pipeline for generating predictions for the selective targeting of microbial groups based on processing assembled and annotated metagenomics data is available at .Conclusions This research demonstrates how genomic-based algorithms can be used to formulate testable hypotheses for strategically engineering the rhizosphere microbiome by identifying specific compounds, which may act as selective modulators of microbial communities. Applying this framework to reduce unpredictable elements in amendment-based solutions promotes the development of ecologically-sound methods for re-establishing a functional microbiome in agro and other ecosystems
A framework for the targeted recruitment of crop-beneficial soil taxa based on network analysis of metagenomics data
Abstract Background The design of ecologically sustainable and plant-beneficial soil systems is a key goal in actively manipulating root-associated microbiomes. Community engineering efforts commonly seek to harness the potential of the indigenous microbiome through substrate-mediated recruitment of beneficial members. In most sustainable practices, microbial recruitment mechanisms rely on the application of complex organic mixtures where the resources/metabolites that act as direct stimulants of beneficial groups are not characterized. Outcomes of such indirect amendments are unpredictable regarding engineering the microbiome and achieving a plant-beneficial environment. Results This study applied network analysis of metagenomics data to explore amendment-derived transformations in the soil microbiome, which lead to the suppression of pathogens affecting apple root systems. Shotgun metagenomic analysis was conducted with data from ‘sick’ vs ‘healthy/recovered’ rhizosphere soil microbiomes. The data was then converted into community-level metabolic networks. Simulations examined the functional contribution of treatment-associated taxonomic groups and linked them with specific amendment-induced metabolites. This analysis enabled the selection of specific metabolites that were predicted to amplify or diminish the abundance of targeted microbes functional in the healthy soil system. Many of these predictions were corroborated by experimental evidence from the literature. The potential of two of these metabolites (dopamine and vitamin B12) to either stimulate or suppress targeted microbial groups was evaluated in a follow-up set of soil microcosm experiments. The results corroborated the stimulant’s potential (but not the suppressor) to act as a modulator of plant beneficial bacteria, paving the way for future development of knowledge-based (rather than trial and error) metabolic-defined amendments. Our pipeline for generating predictions for the selective targeting of microbial groups based on processing assembled and annotated metagenomics data is available at https://github.com/ot483/NetCom2 . Conclusions This research demonstrates how genomic-based algorithms can be used to formulate testable hypotheses for strategically engineering the rhizosphere microbiome by identifying specific compounds, which may act as selective modulators of microbial communities. Applying this framework to reduce unpredictable elements in amendment-based solutions promotes the development of ecologically-sound methods for re-establishing a functional microbiome in agro and other ecosystems. Video Abstrac
Cross-Modal Imaging Reveals Nanoparticle Uptake Dynamics in Hematopoietic Bone Marrow during Inflammation
Nanoparticles have
been employed to elucidate the innate immune
cell biology and trace cells accumulating at inflammation sites. Inflammation
prompts innate immune cells, the initial responders, to undergo rapid
turnover and replenishment within the hematopoietic bone marrow. Yet,
we currently lack a precise understanding of how inflammation affects
cellular nanoparticle uptake at the level of progenitors of innate
immune cells in the hematopoietic marrow. To bridge this gap, we aimed
to develop imaging tools to explore the uptake dynamics of fluorescently
labeled cross-linked iron oxide nanoparticles in the bone marrow niche
under varying degrees of inflammation. The inflammatory models included
mice that received intramuscular lipopolysaccharide injections to
induce moderate inflammation and streptozotocin-induced diabetic mice
with additional intramuscular lipopolysaccharide injections to intensify
inflammation. In vivo magnetic resonance imaging (MRI) and fluorescence
imaging revealed an elevated level of nanoparticle uptake at the bone
marrow as the levels of inflammation increased. The heightened uptake
of nanoparticles within the inflamed marrow was attributed to enhanced
permeability and retention with increased nanoparticle intake by
hematopoietic progenitor cells. Moreover, intravital microscopy showed
increased colocalization of nanoparticles within slowly patrolling
monocytes in these inflamed hematopoietic marrow niches. Our discoveries
unveil a previously unknown role of the inflamed hematopoietic marrow
in enhanced storage and rapid deployment of nanoparticles, which can
specifically target innate immune cells at their production site during
inflammation. These insights underscore the critical function of the
hematopoietic bone marrow in distributing iron nanoparticles to innate
immune cells during inflammation. Our findings offer diagnostic and
prognostic value, identifying the hematopoietic bone marrow as an
imaging biomarker for early detection in inflammation imaging, advancing
personalized clinical care
Cross-Modal Imaging Reveals Nanoparticle Uptake Dynamics in Hematopoietic Bone Marrow during Inflammation
Nanoparticles have
been employed to elucidate the innate immune
cell biology and trace cells accumulating at inflammation sites. Inflammation
prompts innate immune cells, the initial responders, to undergo rapid
turnover and replenishment within the hematopoietic bone marrow. Yet,
we currently lack a precise understanding of how inflammation affects
cellular nanoparticle uptake at the level of progenitors of innate
immune cells in the hematopoietic marrow. To bridge this gap, we aimed
to develop imaging tools to explore the uptake dynamics of fluorescently
labeled cross-linked iron oxide nanoparticles in the bone marrow niche
under varying degrees of inflammation. The inflammatory models included
mice that received intramuscular lipopolysaccharide injections to
induce moderate inflammation and streptozotocin-induced diabetic mice
with additional intramuscular lipopolysaccharide injections to intensify
inflammation. In vivo magnetic resonance imaging (MRI) and fluorescence
imaging revealed an elevated level of nanoparticle uptake at the bone
marrow as the levels of inflammation increased. The heightened uptake
of nanoparticles within the inflamed marrow was attributed to enhanced
permeability and retention with increased nanoparticle intake by
hematopoietic progenitor cells. Moreover, intravital microscopy showed
increased colocalization of nanoparticles within slowly patrolling
monocytes in these inflamed hematopoietic marrow niches. Our discoveries
unveil a previously unknown role of the inflamed hematopoietic marrow
in enhanced storage and rapid deployment of nanoparticles, which can
specifically target innate immune cells at their production site during
inflammation. These insights underscore the critical function of the
hematopoietic bone marrow in distributing iron nanoparticles to innate
immune cells during inflammation. Our findings offer diagnostic and
prognostic value, identifying the hematopoietic bone marrow as an
imaging biomarker for early detection in inflammation imaging, advancing
personalized clinical care
Cross-Modal Imaging Reveals Nanoparticle Uptake Dynamics in Hematopoietic Bone Marrow during Inflammation
Nanoparticles have
been employed to elucidate the innate immune
cell biology and trace cells accumulating at inflammation sites. Inflammation
prompts innate immune cells, the initial responders, to undergo rapid
turnover and replenishment within the hematopoietic bone marrow. Yet,
we currently lack a precise understanding of how inflammation affects
cellular nanoparticle uptake at the level of progenitors of innate
immune cells in the hematopoietic marrow. To bridge this gap, we aimed
to develop imaging tools to explore the uptake dynamics of fluorescently
labeled cross-linked iron oxide nanoparticles in the bone marrow niche
under varying degrees of inflammation. The inflammatory models included
mice that received intramuscular lipopolysaccharide injections to
induce moderate inflammation and streptozotocin-induced diabetic mice
with additional intramuscular lipopolysaccharide injections to intensify
inflammation. In vivo magnetic resonance imaging (MRI) and fluorescence
imaging revealed an elevated level of nanoparticle uptake at the bone
marrow as the levels of inflammation increased. The heightened uptake
of nanoparticles within the inflamed marrow was attributed to enhanced
permeability and retention with increased nanoparticle intake by
hematopoietic progenitor cells. Moreover, intravital microscopy showed
increased colocalization of nanoparticles within slowly patrolling
monocytes in these inflamed hematopoietic marrow niches. Our discoveries
unveil a previously unknown role of the inflamed hematopoietic marrow
in enhanced storage and rapid deployment of nanoparticles, which can
specifically target innate immune cells at their production site during
inflammation. These insights underscore the critical function of the
hematopoietic bone marrow in distributing iron nanoparticles to innate
immune cells during inflammation. Our findings offer diagnostic and
prognostic value, identifying the hematopoietic bone marrow as an
imaging biomarker for early detection in inflammation imaging, advancing
personalized clinical care
Cross-Modal Imaging Reveals Nanoparticle Uptake Dynamics in Hematopoietic Bone Marrow during Inflammation
Nanoparticles have
been employed to elucidate the innate immune
cell biology and trace cells accumulating at inflammation sites. Inflammation
prompts innate immune cells, the initial responders, to undergo rapid
turnover and replenishment within the hematopoietic bone marrow. Yet,
we currently lack a precise understanding of how inflammation affects
cellular nanoparticle uptake at the level of progenitors of innate
immune cells in the hematopoietic marrow. To bridge this gap, we aimed
to develop imaging tools to explore the uptake dynamics of fluorescently
labeled cross-linked iron oxide nanoparticles in the bone marrow niche
under varying degrees of inflammation. The inflammatory models included
mice that received intramuscular lipopolysaccharide injections to
induce moderate inflammation and streptozotocin-induced diabetic mice
with additional intramuscular lipopolysaccharide injections to intensify
inflammation. In vivo magnetic resonance imaging (MRI) and fluorescence
imaging revealed an elevated level of nanoparticle uptake at the bone
marrow as the levels of inflammation increased. The heightened uptake
of nanoparticles within the inflamed marrow was attributed to enhanced
permeability and retention with increased nanoparticle intake by
hematopoietic progenitor cells. Moreover, intravital microscopy showed
increased colocalization of nanoparticles within slowly patrolling
monocytes in these inflamed hematopoietic marrow niches. Our discoveries
unveil a previously unknown role of the inflamed hematopoietic marrow
in enhanced storage and rapid deployment of nanoparticles, which can
specifically target innate immune cells at their production site during
inflammation. These insights underscore the critical function of the
hematopoietic bone marrow in distributing iron nanoparticles to innate
immune cells during inflammation. Our findings offer diagnostic and
prognostic value, identifying the hematopoietic bone marrow as an
imaging biomarker for early detection in inflammation imaging, advancing
personalized clinical care
Cross-Modal Imaging Reveals Nanoparticle Uptake Dynamics in Hematopoietic Bone Marrow during Inflammation
Nanoparticles have
been employed to elucidate the innate immune
cell biology and trace cells accumulating at inflammation sites. Inflammation
prompts innate immune cells, the initial responders, to undergo rapid
turnover and replenishment within the hematopoietic bone marrow. Yet,
we currently lack a precise understanding of how inflammation affects
cellular nanoparticle uptake at the level of progenitors of innate
immune cells in the hematopoietic marrow. To bridge this gap, we aimed
to develop imaging tools to explore the uptake dynamics of fluorescently
labeled cross-linked iron oxide nanoparticles in the bone marrow niche
under varying degrees of inflammation. The inflammatory models included
mice that received intramuscular lipopolysaccharide injections to
induce moderate inflammation and streptozotocin-induced diabetic mice
with additional intramuscular lipopolysaccharide injections to intensify
inflammation. In vivo magnetic resonance imaging (MRI) and fluorescence
imaging revealed an elevated level of nanoparticle uptake at the bone
marrow as the levels of inflammation increased. The heightened uptake
of nanoparticles within the inflamed marrow was attributed to enhanced
permeability and retention with increased nanoparticle intake by
hematopoietic progenitor cells. Moreover, intravital microscopy showed
increased colocalization of nanoparticles within slowly patrolling
monocytes in these inflamed hematopoietic marrow niches. Our discoveries
unveil a previously unknown role of the inflamed hematopoietic marrow
in enhanced storage and rapid deployment of nanoparticles, which can
specifically target innate immune cells at their production site during
inflammation. These insights underscore the critical function of the
hematopoietic bone marrow in distributing iron nanoparticles to innate
immune cells during inflammation. Our findings offer diagnostic and
prognostic value, identifying the hematopoietic bone marrow as an
imaging biomarker for early detection in inflammation imaging, advancing
personalized clinical care
Cross-Modal Imaging Reveals Nanoparticle Uptake Dynamics in Hematopoietic Bone Marrow during Inflammation
Nanoparticles have
been employed to elucidate the innate immune
cell biology and trace cells accumulating at inflammation sites. Inflammation
prompts innate immune cells, the initial responders, to undergo rapid
turnover and replenishment within the hematopoietic bone marrow. Yet,
we currently lack a precise understanding of how inflammation affects
cellular nanoparticle uptake at the level of progenitors of innate
immune cells in the hematopoietic marrow. To bridge this gap, we aimed
to develop imaging tools to explore the uptake dynamics of fluorescently
labeled cross-linked iron oxide nanoparticles in the bone marrow niche
under varying degrees of inflammation. The inflammatory models included
mice that received intramuscular lipopolysaccharide injections to
induce moderate inflammation and streptozotocin-induced diabetic mice
with additional intramuscular lipopolysaccharide injections to intensify
inflammation. In vivo magnetic resonance imaging (MRI) and fluorescence
imaging revealed an elevated level of nanoparticle uptake at the bone
marrow as the levels of inflammation increased. The heightened uptake
of nanoparticles within the inflamed marrow was attributed to enhanced
permeability and retention with increased nanoparticle intake by
hematopoietic progenitor cells. Moreover, intravital microscopy showed
increased colocalization of nanoparticles within slowly patrolling
monocytes in these inflamed hematopoietic marrow niches. Our discoveries
unveil a previously unknown role of the inflamed hematopoietic marrow
in enhanced storage and rapid deployment of nanoparticles, which can
specifically target innate immune cells at their production site during
inflammation. These insights underscore the critical function of the
hematopoietic bone marrow in distributing iron nanoparticles to innate
immune cells during inflammation. Our findings offer diagnostic and
prognostic value, identifying the hematopoietic bone marrow as an
imaging biomarker for early detection in inflammation imaging, advancing
personalized clinical care
Cross-Modal Imaging Reveals Nanoparticle Uptake Dynamics in Hematopoietic Bone Marrow during Inflammation
Nanoparticles have
been employed to elucidate the innate immune
cell biology and trace cells accumulating at inflammation sites. Inflammation
prompts innate immune cells, the initial responders, to undergo rapid
turnover and replenishment within the hematopoietic bone marrow. Yet,
we currently lack a precise understanding of how inflammation affects
cellular nanoparticle uptake at the level of progenitors of innate
immune cells in the hematopoietic marrow. To bridge this gap, we aimed
to develop imaging tools to explore the uptake dynamics of fluorescently
labeled cross-linked iron oxide nanoparticles in the bone marrow niche
under varying degrees of inflammation. The inflammatory models included
mice that received intramuscular lipopolysaccharide injections to
induce moderate inflammation and streptozotocin-induced diabetic mice
with additional intramuscular lipopolysaccharide injections to intensify
inflammation. In vivo magnetic resonance imaging (MRI) and fluorescence
imaging revealed an elevated level of nanoparticle uptake at the bone
marrow as the levels of inflammation increased. The heightened uptake
of nanoparticles within the inflamed marrow was attributed to enhanced
permeability and retention with increased nanoparticle intake by
hematopoietic progenitor cells. Moreover, intravital microscopy showed
increased colocalization of nanoparticles within slowly patrolling
monocytes in these inflamed hematopoietic marrow niches. Our discoveries
unveil a previously unknown role of the inflamed hematopoietic marrow
in enhanced storage and rapid deployment of nanoparticles, which can
specifically target innate immune cells at their production site during
inflammation. These insights underscore the critical function of the
hematopoietic bone marrow in distributing iron nanoparticles to innate
immune cells during inflammation. Our findings offer diagnostic and
prognostic value, identifying the hematopoietic bone marrow as an
imaging biomarker for early detection in inflammation imaging, advancing
personalized clinical care