Percentages (± SE) of 19 salicinoids from greenhouse (solid bars) and field (open bars) grown <i>Populus tremula</i> trees from the SwAsp collection of the four identified chemotypes, CN (2′-cinnamoyl), AC (2′-acetyl), CN-AC (2′-cinnamoyl/2′-acetyl), and TL (<i>tremuloides</i>-like)].

<p>I1 and I2 =  isomer 1 and 2, respectively, based upon UHPLC retention times.</p

Relationship between the two most abundant salicinoids, tremulacin and salicortin, in field grown <i>Populus tremula</i> trees for chemotypes low (TL; solid circles) or high (CN; open circles) in 2′-cinnamoylsalicortin.

<p>Relationship between the two most abundant salicinoids, tremulacin and salicortin, in field grown <i>Populus tremula</i> trees for chemotypes low (TL; solid circles) or high (CN; open circles) in 2′-cinnamoylsalicortin.</p

Structural relationship of 19 salicinoids found in the foliage of <i>Populus tremula</i> from the SwAsp collection grouped similar to the loading plot (Fig. 2b).

<p>1 =  new compounds for <i>P. tremula</i>, 2 =  compounds with two isomers present but the conformation of the cinnamoyl group double bond is ambiguous. 3 =  2′-(<i>E</i>)- and 2′-(<i>Z</i>)-cinnamoylsalicortin.</p

UV maxima, theoretical and experimental exact masses, molecular formulas, and main high-resolution MS/MS fragments of the new salicinoids from <i>Populus tremula</i>.

<p>See Fig. S1a in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107189#pone.0107189.s001" target="_blank">Material S1</a> for MS/MS spectra.</p><p>a =  MS/MS performed on deprotonated isomer 2 of cinnamoyl compounds, except for cinnamoylsalicin, which used the formate adduct [M+FA-H]^- (experimental mass <i>m/z</i> 461.1433) of isomer 2; b =  isomer 1, c =  isomer 2 by UHPLC retention times.</p><p>UV maxima, theoretical and experimental exact masses, molecular formulas, and main high-resolution MS/MS fragments of the new salicinoids from <i>Populus tremula</i>.</p

Salicinoid chemotypes of <i>Populus tremula</i> trees (the SwAsp collection) collected from ten locations throughout Sweden.

<p>White  =  CN (2′-cinnamoyl), light gray  =  AC (2′-acetyl), dark gray  =  CN-AC (2′-cinnamoyl/2′-acetyl), and black  =  TL (<i>tremuloides</i>-like). Bar height corresponds to the number of individuals of each chemotype.</p

<i>F</i> and <i>P</i> values from the two-factor ANOVA comparing percentages of individual leaf extract salicinoids from <i>Populus tremula</i> trees of four chemotypes (CN, AC, CN-AC, and TL), grown in two environments (greenhouse and field), and their interaction (E*Ct).

<p><i>I</i>1 and <i>I</i>2 indicate isomers 1 and 2, respectively, designated by UHPLC retention times. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107189#pone-0107189-g003" target="_blank">Figure 3</a> for corresponding data.</p><p><i>F</i> and <i>P</i> values from the two-factor ANOVA comparing percentages of individual leaf extract salicinoids from <i>Populus tremula</i> trees of four chemotypes (CN, AC, CN-AC, and TL), grown in two environments (greenhouse and field), and their interaction (E*Ct).</p

PCA results for chemotypes and compound relationship of 19 salicinoids found in the foliage of <i>Populus tremula</i> from the SwAsp collection. a.

<p>Score scatter plot of the first two principle components for 319 individuals from 102 genotypes. Solid symbols  =  greenhouse grown trees, open symbols  =  field grown trees; circles  =  CN (2′-cinnamoyl), diamonds  =  AC (2′-acetyl), squares  =  CN-AC (2′-cinnamoyl/2′-acetyl), and triangles  =  TL (<i>tremuloides</i>-like) chemotypes. Percentages of clones of the different salicinoid chemotypes in parentheses. <b>b.</b> Loading scatter plot of the first two principle components for 19 salicinoids.</p

Clonal repeatabilities (<i>H</i>²) of 19 salicinoids from 85 clones of <i>Populus tremula</i> from greenhouse (GH), field, and combined (All) populations. <i>I</i>1 and <i>I</i>2 indicate isomers 1 and 2, designated by UHPLC retention times.

<p>Clonal repeatabilities (<i>H</i>²) of 19 salicinoids from 85 clones of <i>Populus tremula</i> from greenhouse (GH), field, and combined (All) populations. <i>I</i>1 and <i>I</i>2 indicate isomers 1 and 2, designated by UHPLC retention times.</p

Additional file 5: of A multi-omics approach reveals function of Secretory Carrier-Associated Membrane Proteins in wood formation of​ ​​Populus​​ ​trees

LC-MS metabolomic analysis of wild type and PttSCAMP3 RNAi plants. (XLSX 64 kb

Additional file 3: of A multi-omics approach reveals function of Secretory Carrier-Associated Membrane Proteins in wood formation of​ ​​Populus​​ ​trees

RNAseq analysis of wild type and PttSCAMP3 RNAi plants. (XLSX 1841 kb