Additional file 1 of The importance of meropenem resistance, rather than imipenem resistance, in defining carbapenem-resistant Enterobacterales for public health surveillance: an analysis of national population-based surveillance

Additional file 1. Supplementary material 1

Phylogenetic tree of the whole amino acid sequences of chaperonin GroES homologs.

<p>Detailed analysis is same as <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025334#pone-0025334-g003" target="_blank">Fig. 3</a>.</p

Schematic representation of multiple drug-resistance determinants.

<p>Pairwise comparison of plasmid regions around the <i>bla</i>_NDM-1 gene in pNDM-1_Dok01, pNDM-HK, and pKpANDM-1 in <i>K. pneumoniae</i> KP-05-506 and <i>E. coli</i> strain 271 by a BLASTN homology search and visualized with the ACT program. The <i>bla</i>_NDM-1 genes are identical among the aligned sequences. The red and blue bars between the DNA represent individual nucleotide matches in the forward and inverted directions, respectively. BLASTN match scores of <300 are not shown.</p

ORFs in NDM-1 composite transposon.

<p>ORFs in NDM-1 composite transposon.</p

Circular representation of the <i>E. coli</i> NDM-1_Dok01 plasmid pNDM-1_Dok01.

<p>From the outside inwards, the outer circle indicates the homologous regions to the <i>E. coli</i> strain AR060302 plasmid pAR060302 (red) and <i>E. coli</i> strain HK-01 plasmid pNDM-HK (orange). The second circle shows the size in base pairs (bp). The third and fourth circles show the positions of the CDSs transcribed in the clockwise and anti-clockwise directions, respectively (using color codes according to the clusters of orthologous groups (COG) classification table and additional customized categories). The fifth circle shows a plot of the G + C content (in 0.5 kb windows).</p

Phylogenetic tree of the whole amino acid sequences of chaperonin GroEL homologs.

<p>The amino acid sequences were selected and retrieved with a BLASTP search against the refseq_protein database with a cut-off value of 75% identity. The tree was constructed using the maximum likelihood method with 1,000 bootstrap replicates. The scale indicates that a branch length of 0.03 is 3 times as long as one that would show a 1% difference between the amino acid sequences at the beginning and end of the branch. The number at each branch node represents the bootstrapping value. The chromosomal GroEL in <i>E. coli</i> NDM-1_Dok01 is highlighted in blue. The GC percentage of the respective nucleotide sequences is shown on the right-hand side of the figure.</p