1 research outputs found
Bioinspired Quercitrin Nanocoatings: A Fluorescence-Based Method for Their Surface Quantification, and Their Effect on Stem Cell Adhesion and Differentiation to the Osteoblastic Lineage
Polyphenol-based
coatings have several potential applications in medical devices, such
as cardiovascular stents, contrast agents, drug delivery systems,
or bone implants, due to the multiple bioactive functionalities of
these compounds. In a previous study, we fabricated titanium surfaces
functionalized with flavonoids through covalent chemistry, and observed
their osteogenic, anti-inflammatory, and antifibrotic properties in
vitro. In this work, we report a fluorescence-based method for the
quantification of the amount of flavonoid grafted onto the surfaces,
using 2-aminoethyl diphenylborinate, a boronic ester that spontaneously
forms a fluorescent complex with flavonoids. The method is sensitive,
simple, rapid, and easy to perform with routine equipment, and could
be applied to determine the surface coverage of other plant-derived
polyphenol-based coatings. Besides, we evaluated an approach based
on reductive amination to covalently graft the flavonoid quercitrin
to Ti substrates, and optimized the grafting conditions. Depending
on the reaction conditions, the amount of quercitrin grafted was between
64 ± 10 and 842 ± 361 nmol on 6.2 mm Ti coins. Finally,
we evaluated the in vitro behavior of bone-marrow-derived human mesenchymal
stem cells cultured on the quercitrin nanocoated Ti surfaces. The
surfaces functionalized with quercitrin showed a faster stem cell
adhesion than control surfaces, probably due to the presence of the
catechol groups of quercitrin on the surfaces. A rapid cell adhesion
is crucial for the successful performance of an implant. Furthermore,
quercitrin-nanocoated surfaces enhanced the mineralization of the
cells after 21 days of cell culture. These results indicate that quercitrin
nanocoatings could promote the rapid osteointegration of bone implants