rs6917 allele-specific <i>PHB</i> expression.

<p>A) The log₁₀ of FAM/VIC intensity ratio for <i>PHB</i> plotted against the log₁₀ of FAM/VIC allele ratio of mixing homozygous DNAs at different ratios (8∶1, 4∶1, 2∶1, 1∶1, 1∶2, 1∶4, 1∶8 FAM/VIC allele). B) <i>PHB</i> expression by rs6917 genotype in gastric samples. C) C/T allelic ratio in cDNA from gastric samples of heterozygous patients by sequencing; D) FAM/VIC allelic ratio in gastric samples of heterozygous patients using a Custom Genotyping TaqMan assay, in which a specific probe for the C allele was labeled with FAM dye and a minor allele T probe was labeled with VIC dye. *Differentially expressed between groups by the T-test for independent samples, <i>P</i><0.05.</p

Immunohistochemical analysis of PHB in gastric samples.

<p>A) PHB staining in normal gastric mucosa (400x); B) PHB immunoreactivity in normal gastric mucosa and inflammatory cells (400x); C) strong PHB staining in intestinal metaplastic cells (400x); D) strong PHB staining in an intestinal-type tumor; E) moderate to intense PHB immunoreactivity in a poorly differentiated tumor; F) moderate to intense PHB immunoreactivity in a moderately differentiated tumor; G) weak PHB staining in a moderately differentiated tumor (400x); D) weak PHB staining in a diffuse-type tumor (400x).</p

Clinicopathological characteristics and <i>PHB</i> expression in gastric cancer samples.

<p>N: number of individuals; SD: standard deviation.</p>a<p>Number of samples after outlier exclusion;</p>b<p>According to Laurén <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098583#pone.0098583-Lauren1" target="_blank">[22]</a>;</p>c<p>According to TNM staging <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098583#pone.0098583-Washington1" target="_blank">[23]</a>;</p>d<p>Differentially expressed between groups by T-test for independent samples, <i>P</i><0.05.</p

Clinicopathological characteristics and <i>PHB</i> copy number in gastric cancer samples.

<p>N: number of individuals;</p>a<p>According to Laurén <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098583#pone.0098583-Lauren1" target="_blank">[22]</a>;</p>b<p>According to TNM staging <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098583#pone.0098583-Washington1" target="_blank">[23]</a>;</p>c<p>Differentially expressed between groups by Chi-square test or Fisher’s exact test, <i>P</i><0.05.</p

<i>PHB</i> mRNA expression by copy number.

<p>Lines show the median and interquartile range of <i>PHB</i> expression. *Differentially expressed between groups by the Mann-Whitney test, <i>P</i><0.05.</p

Immunohistochemistry analysis in gastric tumors.

a<p>According to Laurén <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098583#pone.0098583-Lauren1" target="_blank">[22]</a>;</p>b<p>According to TNM staging <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0098583#pone.0098583-Washington1" target="_blank">[23]</a>.</p