Additional file 1: of Expression of human CD46 and trans-complementation by murine adenovirus 1 fails to allow productive infection by a group B oncolytic adenovirus in murine cancer cells

Immunoblotting of FLAG-tagged MAV1 ORF transgenes encoded in EnAd. NMuMG-CD46 cells were infected with EnAd encoding different MAV1 ORFs as CMV-driven FLAG-tagged transgenes. A. Three days post-infection, cells were lysed and probed for the presence of the FLAG tag using a horseradish peroxidase-conjugated mouse anti-DYKDDDDK antibody. Red asterisks above bands denote proteins of approximately the predicted size for each transgene. Blots were probed with horseradish peroxidase-conjugated mouse anti-β-actin as a loading control. B. Predicted protein sizes for each ORF. (PPTX 1369 kb