The technique of the factorial analysis of the profitability of the segments in waterborn transport companies

The present article substantiates the need for carrying out a factor analysis of the profitability of the operations of the segments in water-born transport companies. The stages of the factor analysis of profitability have been defined with the factors affecting both the profitability of spending of one segmentandthe overall profitability of spending having been determined as well. In addition, the factors affecting the profitability of watercraft have been studied. The proposed method of performing the factor analysis of profitability is examinedthrough the example of AltynYar, an organization engaged in water-borne transportation. The conclusions that were drawn following the completion of the factor analysis suggest that employing the analysis allows determining the impact of specific causes upon the results of the segments' activitiesand contributes to the identification and further elimination of negative trends

Analysis of Genome Grimelysin-Containing Locus in the Genome of Serratia grimesii A2

© 2016, Springer Science+Business Media New York.Serratia grimesii strain A2 was isolated from buffered solution of actin by workers of Institute of Marine Biology, Vladivostok city [1]. This bacterium attracts special interest because of its ability to produce intracellular proteinase grimelysin which hydrolyzes actin in the single site and thus abolishes actin polymerization [1]. In addition to this well-characterized function, grimelysin is also required for invasion of bacteria into eukaryotic cells [1]. Here, we present the analysis of genome sequence of S. grimesii strain A2. The 5133.068 bp draft genome consists of 120 contigs with 4999 coding sequences, including 82 RNA genes. Genome sequencing and annotation revealed the presence of grimelysin gene in the operon with the gene of hypothetical protein of unknown function and the presence of regulatory gene upstream of it. In silico analysis of promoter sequences allowed the identification of putative binding sites for regulatory proteins. We anticipate that the S. grimesii strain A2 genome analysis will advance the current knowledge about grimelysin functions and regulation of its expression

Spoken-behavioral analysis of the poem by M.Y. Lermontov "song about Tsar Ivan Vasilyevich, a young oprichnik and brave merchant Kalashnikov"

Song about Tsar Ivan Vasilyevich, a young oprichnik and brave merchant Kalashnikov had drawn attention of many scientists [Lominadze, Golstein, Kiseleva, Komar], who were considering it in literary key, at this - in different aspects, including religious, detecting contradictions in estimation of Christian attitude of the poet. Along with that, the poem of M.Y. Lermontov is interesting from language culturological positions, as language has an ability to accumulate culture (including religious) of people speaking it. Methods of research are description and language culturological. Research was conducted with reliance on conception of spokenbehavioral tactics of E.M. Vereshchagin and V.G. Kostomarov and figured by them spoken-behavioral tactics of the central fragment of Russian national culture - the culture of guilt and apology. In work are analyzed spoken-behavioral tactics of delict (guilt of any kind) admission and its obliteration and also their verbal realizations. There were separated spoken-behavioral tactics of forced guilt admission, guilt admission and also singular and abstinative spoken-behavioral tactics showing divergence of world vision of M.Y. Lermontov with ethic of the New Testament. In result was made a conclusion abut the fact that setting for religiousness of M.Y. Lermontov in considered poem does not correspond to truly Christian school of thought

Cloning and Expression of a Metalloprotease Gene from Morganella morganii Strain ZM

© 2017, Springer Science+Business Media, LLC. Morganella morganii is an opportunistic pathogen which causes a wide range of clinical infections. It is well known that metalloproteases synthesized by pathogenic microorganisms play an important role as virulence factors. In this work, we have conducted bioinformatic analysis, sequencing, and cloning of the gene encoding a putative metalloprotease from M. morganii (AN CP004345.1, MU9_1718). We detected that the amino acid sequence of putative metalloprotease from M. morganii strain KT has 37% identity to actin-specific metalloproteases grimelysin and protealysin from Serratia grimesii and Serratia proteamaculans, respectively. Based on ORF MU9_1718 sequence, we have identified a metalloprotease gene in the genome of clinical isolate M. morganii ZM. The gene was cloned under the control of arabinose promoter into pBAD/Myc-His expression vector, and Escherichia coli DH5α cells were subsequently transformed by this vector. We also provide data indicating that the metalloprotease was overexpressed in E. coli DH5α cells as a 35 kDa protein

Identification of Grimelysin-Like Metalloprotease Gene in the Genome of Bacterium Providencia stuartii

© 2016, Springer Science+Business Media New York.Providencia stuartii is an opportunistic pathogen often seen in patients with severe burns or long-term indwelling urinary catheters. Nowadays, the clinical significance of opportunistic microorganisms is growing and the study of their pathogenesis mechanisms is necessary. Microbial proteases are recognized as important virulence factors of diverse bacterial pathogens. We have shown that P. stuartii’s bacterial extracts have the ability to cleave actin. It is well known that metalloprotease grimelysin from Serratia grimesii is characterized by high specificity towards actin. Using the BLAST program, we identified a gene of hypothetical metalloprotease in the genome of the annotated strain P. stuartii MRSN 2154. We have constructed gene-specific primers and sequenced a homologous metalloprotease gene from clinical isolate P. stuartii NK. The amino acid sequence of this gene has the 42 % identity with grimelysin metalloprotease. In this study, we have done a comparative analysis of this novel protease from the clinical isolate of P. stuartii NK with the grimelysin from S. grimesii A2

Virulence factors contributing to invasive activities of Serratia grimesii and Serratia proteamaculans

© 2015, Springer-Verlag Berlin Heidelberg. Previously, we have shown that facultative pathogens Serratia grimesii and Serratia proteamaculans are capable to invade eukaryotic cells provided that they synthesize intracellular metalloprotease grimelysin or protealysin, respectively (Bozhokina et al. in Cell Biol Int 35(2):111–118, 2011). Noninvasive Escherichia coli transformed with grimelysin or protealysin gene became invasive, indicating that the protease is a virulence factor. Here we elucidated involvement of other virulence factors in the invasion of S. grimesii and S. proteamaculans. Under similar experimental conditions, the amount of S. proteamaculans internalized within human carcinoma HeLa cells was fivefold higher than that of S. grimesii. In accord with this, in S. proteamaculans, high activities of pore-forming hemolysin ShlA and extracellular metalloprotease serralysin were detected. In S. grimesii, activity of toxin ShlA was not detected, and the serralysin activity of the bacterial growth medium was very low. We also show that iron depletion strongly enhanced invasive activity of S. proteamaculans, increasing activities of hemolysin ShlA and serralysin, but did not affect S. grimesii properties. These results show that the invasive activity of S. proteamaculans is maintained, along with protealysin, by hemolysin and serralysin. On the other hand, grimelysin is so far the only known invasion factor of S.grimesii

Spoken-behavioral analysis of the poem by M.Y. Lermontov "song about Tsar Ivan Vasilyevich, a young oprichnik and brave merchant Kalashnikov"

Song about Tsar Ivan Vasilyevich, a young oprichnik and brave merchant Kalashnikov had drawn attention of many scientists [Lominadze, Golstein, Kiseleva, Komar], who were considering it in literary key, at this - in different aspects, including religious, detecting contradictions in estimation of Christian attitude of the poet. Along with that, the poem of M.Y. Lermontov is interesting from language culturological positions, as language has an ability to accumulate culture (including religious) of people speaking it. Methods of research are description and language culturological. Research was conducted with reliance on conception of spokenbehavioral tactics of E.M. Vereshchagin and V.G. Kostomarov and figured by them spoken-behavioral tactics of the central fragment of Russian national culture - the culture of guilt and apology. In work are analyzed spoken-behavioral tactics of delict (guilt of any kind) admission and its obliteration and also their verbal realizations. There were separated spoken-behavioral tactics of forced guilt admission, guilt admission and also singular and abstinative spoken-behavioral tactics showing divergence of world vision of M.Y. Lermontov with ethic of the New Testament. In result was made a conclusion abut the fact that setting for religiousness of M.Y. Lermontov in considered poem does not correspond to truly Christian school of thought

Place of nature in economic development

In the presence of externalities involving nature's services, individuals and communities overexploit natural capital. In other words ecological services are subsidized. This working paper proposes a mathematical model that factors in values of social and environmental costs/benefits. Nature consists of degradable resources: resource stocks are self-regenerative, but suffer from depletion or deterioration when they are over-used. Extreme poverty is frequently associated with a degraded environment. Studies confirm that the world's poorest people live in especially fragile natural environments. When policies are evaluated a social cost-benefit analysis must be included

New metalloendopeptidase of Morganella morganii ZM

© 2014 Pleiades Publishing, Ltd. Proteolytic activity, which is inhibited in the presence of o-phenanthroline, was found in M. morganii ZM. Unlike grimelysin, intracellular proteases of M. morganii ZM actively cleave skeletal muscle actin. Several proteolytic proteins of M. morganii ZM were identified in cells by zymography with gelatin. Individual metalloproteinase (35 kDa) was isolated from the M. morganii ZM cell lysates by ammonium sulphate fractionation and purified by hydrophobic chromatography

Efflux systems in Serratia marcescens

A widespread bacterium Serratia marcescens (family Enterobacteriaceae) is an opportunistic pathogen and exhibits multiple drug resistance. Active removal of antibiotics and other antimicrobials from the cells by efflux systems is one of the mechanisms responsible for microbial resistance to these compounds. Among enterobacteria, efflux systems of Escherichia coli and Salmonella enterica ser. Typhimurium have been studied most extensively. Few efflux systems that belong to different families have been reported for S. marcescens. In this review, we analyzed available literature about S. marcescens efflux systems and carried out the comparative analysis of the genes encoding the RND type systems in different Serratia species and in other enterobacteria. Bioinformatical analysis of the S. marcescens genome allowed us to identify the previously unknown efflux systems based on their homology with the relevant E. coli genes. Identification of additional efflux systems in S. marcescens genome will promote our understanding of the physiology of these bacteria, will detect new molecular mechanisms of resistance, and will reveal their resistance potential. © 2013 Pleiades Publishing, Ltd