Univariate analysis (<i>p</i><0.25) according to the characteristics of the dogs (<i>n</i> = 455), Brazil 2010.

<p>Abbreviations: LZOON, Laboratory of Zoonosis of the Belo Horizonte Health Department; LIMP, Laboratory of Immunopathology of the Federal University of Ouro Preto; HR, hazard ratio; CI, confidence interval.</p

Baseline survey; evaluations I, II, and III; and losses to follow-up, Belo Horizonte, Minas Gerais.

<p>Baseline survey; evaluations I, II, and III; and losses to follow-up, Belo Horizonte, Minas Gerais.</p

Univariate analysis (<i>p</i><0.25) according to the understanding of the disease and environmental conditions, Brazil 2010.

<p>Abbreviations: LZOON, Laboratory of Zoonosis of the Belo Horizonte Health Department; LIMP, Laboratory of Immunopathology of the Federal University of Ouro Preto; HR, hazard ratio; CI, confidence interval.</p

Characteristics of dogs in the cohort study, Brazil 2010.

<p>Characteristics of dogs in the cohort study, Brazil 2010.</p

Dog-months of follow-up, failure events (seroconversion), and incidence rates with 95% CIs, Brazil 2010.

a, b, c<p>10, 16, and 26 months after baseline, respectively;</p>d<p>Incidence rate/1000 dogs-months. Abbreviations: LZOON, Laboratory of Zoonosis of the Belo Horizonte Health Department; LIMP, Laboratory of Immunopathology of the Federal University of Ouro Preto; CI, confidence interval.</p

Distribution of owners (<i>n</i> = 918) of seronegative dogs according to the socioeconomic and environmental conditions and understanding of the disease, Brazil 2008.

<p>*Brazilian minimum wages (Brazilian monthly minimum wage = U$262).</p

Municipality of Belo Horizonte, state of Minas Gerais, Brazil, subdivided into sanitary areas.

<p>Municipality of Belo Horizonte, state of Minas Gerais, Brazil, subdivided into sanitary areas.</p

Distribution of seronegative dogs (<i>n</i> = 1213) according to the characteristics of the animals, Brazil 2008.

<p>Distribution of seronegative dogs (<i>n</i> = 1213) according to the characteristics of the animals, Brazil 2008.</p

Hierarchical framework of risk factors for <i>Leishmania infantum</i> infection in dogs.

<p>Hierarchical framework of risk factors for <i>Leishmania infantum</i> infection in dogs.</p

Cellular profile of circulating lymphocytes and monocytes in dogs submitted to different vaccination protocols before and after challenge with <i>L. infantum</i> plus SGE.

<p>C (control; white square); Sap (saponin; white triagle); LB (killed <i>L. braziliensis</i> vaccine; black square), LBSap (killed <i>L. braziliensis</i> vaccine plus saponin; black triagnle): the <i>x</i>-axis displays the times at which the assays were conducted (Tbc: time before challenge with <i>L</i>. <i>infantum</i>; and 20, 90 274, 435, 541 and 885 days after challenge [dac] with <i>L</i>. <i>infantum</i>), and the <i>y</i>-axis represents the mean values of the (A) absolute counts of circulating lymphocytes and of (B) CD5⁺, (C) CD4⁺, (D) CD8⁺, (E) CD21⁺ cells and (F) CD14⁺ monocytes. Significant differences (<i>p</i><0.05) between the LBSap group and the control C, Sap and LB groups are indicated, respectively, by the letters a, b and c.</p