The acidic intrinsically disordered region of the inflammatory mediator HMGB1 mediates fuzzy interactions with chemokine CXCL12

Data contain: The acidic intrinsically disordered region of the inflammatory mediator HMGB1 mediates fuzzy interactions with chemokine CXCL12 Data contain: Figure 2: data generating bar graphs displayed in b,c,f,g,jk Figure 3: data generating bar graphs displayed in c,d,f,g Figure 4: data generating bar graphs displayed in c-f Figure 5: ITC sequential pulses displayed in Figure 5a-c, normalized MST signals displayed in Figure d,e Figure 6: SV-AUC data displayed in a-c, MS-SV analysis displayed in f Figure 7:SAXS data displayed in a-d Figure 8:Quantification of PLA signals displayed in a-c Supplementary S3: data generating bar graphs displayed in b-k Supplementary Figure S4:data generating bar graphs displayed in b-d; normalized MST signals displayed in e Supplementary Figure S5: data generating bargprahs displayed in panel a,b;d,e;g,h Supplementary Figure S6: ITC sequential pulses displayed in c,d Supplementary Figure S7:SV-AUC data displayed in a-i, MS-SV analysis displayed in k. Supplementary Figure S8: SAXS data displayed in a,b Supplementary Figure S9: HMGB1 SAXS dilution series displayed in a-c Supplementary Figure S10: CXCL12 SAXS dilution series displayed in a-c Supplementary Figure S11 SAXS data and EOM analysis displayed in a and b Figure S12: SAXS concentration series of frHMGB1 titrated with CXCL12 a-b Figure S13: Supplementary Figure S12: Fits to SAXS data of the a) SASREF and b)FoXSDock model of frHMGB1-CXCL12 complex Figure S15: OLIGOMER analysis, panel a,b Figure S16: PLA analysis, panel b Figure S17: uncropped gel in panel a, FACS analysis pnale c Reporting summary: AB1 Cell line information Supplementary Table 1: contains the AUC analysis of CXCL12, frHMGB1 and frHMGB1•CXCL12 at pH 7.5, 20 mM TrisHCl, 50 mM NaCl Supplementary Table 2: contains the calculated values from AUC analysis of CXCL12, frHMGB1 and frHMGB1●CXCL12, pH 7.5, 20 mM TrisHCl,150 mM NaCl Supplementary Table 3: contains the calculated values from AUC analysis of CXCL12-LM, frHMGB1 and frHMGB1●CXCL12-LM, pH 7.5, 20 mM TrisHCl, 50 mM NaC