Average values of the binding constants for the studied systems.

<p><i>Average K values calculated by using the Benesi–Hildebrand equation for all the isophorone-DNA systems studied</i>.</p><p>Average values of the binding constants for the studied systems.</p

Chemical structures.

<p>Structures of: A) Isoa, B) Isob and C) Isoc.</p

Stern-Volmer plots for the fluorescence quenching of Isoa-c by DNA.

<p>Plot of the ratio of the isophorone derivatives fluorescence intensity before and after incremental addition of DNA as a function of the quencher concentration for the determination of the Stern-Volmer quenching rate constant.</p

Effect of the isophorone derivatives on the DNA conformation.

<p>Circular dichroism (CD) spectra of DNA (30.9 μM) treated with: A) 0.0 (black line), 12.82 (red line), 38.07 (blue line), 62.81(green line) μM (curves 1–4) of Isoa, B) 0.0 (black line), 12.82 (red line), 38.07 (blue line), 62.81(green line) μM (curves 1–4) of Isob, C) 0.0 (black line), 12.82 (red line), 38.07 (blue line), 62.81(green line) μM (curves 1–4) of Isoc.</p

Binding constant determination.

<p>Plots of 1/(A-A₀) <i>vs</i>. 1/Isophorone concentration for DNA and their isophorone complexes. A₀ is the absorbance of DNA (at 260 nm) and A is the absorbance after isophorone addition.</p

Binding stoichiometry.

<p>Mole ratio plot of Isoa-DNA system.</p

Plot of log [(F₀-F)/F] <i>vs</i>. log [DNA].

<p>Determination of the binding constant and number of binding sites for the Iso-DNA systems.</p