Additional file 2: Figure S2. of Dendritic cells, macrophages, NK and CD8+ T lymphocytes play pivotal roles in controlling HSV-1 in the trigeminal ganglia by producing IL1-beta, iNOS and granzyme B

Representative FACS density plots showing the gate strategy for the identification of iNOS within F4/80+ gated on live CD45+ leucocytes in the trigeminal ganglia (a) and spleen (b) from a single HSV1-infected WT mouse. A minimum of 100,000 events was acquired for analysis. (PPTX 2700 kb

Additional file 1: Figure S1. of Dendritic cells, macrophages, NK and CD8+ T lymphocytes play pivotal roles in controlling HSV-1 in the trigeminal ganglia by producing IL1-beta, iNOS and granzyme B

Representative FACS density plots showing the gate strategy for the identification of IL-1β within CD11c+MHCIIhigh gated on live CD45+ leucocytes in the trigeminal ganglia (a) and spleen (b) from a single HSV1-infected WT mouse. A minimum of 100,000 events was acquired for analysis. (PPTX 386 kb

Demographic and Plasma VWF for 10,434 ARIC Participants by Race.

<p>include current and former smokers.</p

Influence of relative humidity on fracture toughness of rock: Implications for subcritical crack growth

<p>* <b>Top row: values adjusted for age, smoking, BMI, diabetes, and hypertension and bottom row (shaded): values adjusted for</b> environmental factors as in top row, but also for VWF</p><p>Geometric Mean (95% CI) of FVIII Activity (%) in A Blood Groups^{<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132626#t004fn001" target="_blank">*</a>}.</p

VWF haplotypes.

<p>The haplotypes were constructed using the default setting that (1) ignores pairview comparisons of markers more than 500 kb apart, (2) excludes subjects with more than 50% of missing genotypes, (3) examines haplotypes found 1% or more in the population, (4) removes markers with Hardy-Weinberg disequilibrium, and (5) uses R² to define haplotype blocks.</p

Allele Frequency of VWF SNP in Four Ethnic Groups (%).

<p>Allele Frequency of VWF SNP in Four Ethnic Groups (%).</p

Quantitative Influence of ABO Blood Groups on Factor VIII and Its Ratio to von Willebrand Factor, Novel Observations from an ARIC Study of 11,673 Subjects

<div><p>ABO blood groups are known to influence the plasma level of von Willebrand factor (VWF), but little is known about the relationship between ABO and coagulation factor VIII (FVIII). We analyzed the influence of ABO genotypes on VWF antigen, FVIII activity, and their quantitative relationship in 11,673 participants in the Atherosclerosis Risk in Communities (ARIC) study. VWF, FVIII, and FVIII/VWF levels varied significantly among O, A (A1 and A2), B and AB subjects, and the extent of which varied between Americans of European (EA) and African (AA) descent. We validated a strong influence of ABO blood type on VWF levels (15.2%), but also detected a direct ABO influence on FVIII activity (0.6%) and FVIII/VWF ratio (3.8%) after adjustment for VWF. We determined that FVIII activity changed 0.54% for every 1% change in VWF antigen level. This VWF-FVIII relationship differed between subjects with O and B blood types in EA, AA, and in male, but not female subjects. Variations in FVIII activity were primarily detected at low VWF levels. These new quantitative influences on VWF, FVIII and the FVIII/VWF ratio help understand how ABO genotypes differentially influence VWF, FVIII and their ratio, particularly in racial and gender specific manners.</p></div

Effect Size (%) of Covariates for FVIII activity and FVIII/VWF ratio.

<p>* Semipartial ω² is the proportion of variability explained by each factor.</p><p>^a Model was defined as log FVIII = log VWF + ABO + environmental covariates (age, BMI, hypertension, diabetes, ever smoking status, and combination of race and gender).</p><p>^b Model was defined as log FVIII/VWF ratio = ABO + environmental covariates (age, BMI, hypertension, diabetes, ever smoking status, and combination of race and gender).</p><p>Effect Size (%) of Covariates for FVIII activity and FVIII/VWF ratio.</p

Baseline Characteristics of Subjects in Each ABO Blood Group^‡.

<p>^‡11,673 subjects were included in the study, but for covariates the numbers of subjects were 11,661 for BMI</p><p>^† Age at baseline visit</p><p>* Analysis of variance</p><p>** Chi-square test</p><p>Baseline Characteristics of Subjects in Each ABO Blood Group<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132626#t001fn001" target="_blank">^‡</a>.</p