ELISA reproducibility.

<p>Data from three <i>Opisthorchis</i>-positive (N. 1–3) and six <i>Opisthorchis</i>-negative (N. 4–9) serum samples corresponding to eight different working sessions.</p

Diagnostic sensitivity, specificity, and positive and negative predictive values (cut off 17%, 95% CI) for serum samples from people with a diagnosis of <i>Opisthorchis felineus</i> infection confirmed by the detection of <i>O. felineus</i> eggs in their stool, serum samples from <i>Opisthorchis</i> sp. -free people, and serum samples from people suffering from other parasitic or non-parasitic infections.^a

a<p>Sensitivity = 100% (95% CI: 97.45–100%), it was calculated as TP/(TP+FN); Specificity = 90.27% (95% CI: 85.63–93.80%), it was calculated as TN/(TN+FP);</p>c<p>Positive predictive value = 86.75% (95% CI: 80.62–91.50%), it was calculated as TP/(TP+FP); Negative predictive value = 100% (95% CI: 98.19–100%), it was calculated as TN/(TN+FN). TP, TN, FP, and FN are the numbers of true positives, true negatives, false positives, and false negatives, respectively.</p

Descriptive indices of ELISA results to detect anti-<i>Opisthorchis</i> IgG in sera from persons with a confirmed diagnosis of opisthorchiasis (infected), and from healthy persons (non-infected).

<p>Descriptive indices of ELISA results to detect anti-<i>Opisthorchis</i> IgG in sera from persons with a confirmed diagnosis of opisthorchiasis (infected), and from healthy persons (non-infected).</p

Scatter plot of the differences between the optical density (OD) of the serum duplicates and their means.

<p>OD was recorded at 450 nm.</p