3 research outputs found
Tumor formation, tumor burden and ascites in the OCLER and FNLER xenograft model.
a<p>Values shown are means ± s.d. across all mice injected with each cell type that had any evaluable tumor mass (the sum of 1× intraperitoneal and 2× subcutaneous sites per mouse).</p>b<p>Formation of ascites was only evaluated among mice that developed tumors.</p>c<p><i>P</i>-values were calculated using the Mann-Whitney test.</p
OCLER and FNLER tumor histopathology in immunodeficient nude (Nu/Nu) mice.
<p><b>A-B</b>, Hematoxylin and eosin (H&E) stains of representative formalin-fixed paraffin-embedded (FFPE) tumor sections from OCLER (A) and FNLER (B) xenografts revealed focal micropapillary structures. The predominant morphology was diffuse sheets of cells with a poorly differentiated tumor architecture (scale bar  =  20 µM). <b>C-D</b>, PAX8 immunoperoxidase stains of representative FFPE tumor sections from OCLER (C) and FNLER (D) xenografts confirmed that xenografts retained their PAX8 expression (scale bar  =  20 µM).</p
Culture and characterization of normal ovarian epithelial and fallopian tube epithelial cells.
<p><b>A</b>, Replicate plates of normal fallopian tube epithelial cells were cultured in WIT-fo medium (green and purple lines) or standard medium (red, grey lines, 1:1 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and Ham’s F12, supplemented with 0.1% BSA, 5% serum). In WIT-fo medium, normal fallopian tube epithelial cells from two different patients divided continuously for at least 30 days and > 100 days and reached at least 12 and >20 population doublings respectively (green and purple lines). In contrast, matched cells from the same donors growth arrested in the DMEM/Ham’s F12 medium (red, grey lines). Normal fallopian tube epithelial cells were isolated from additional patients 3 and 4. <b>B</b>, Normal ovarian epithelial cells were cultured in WIT-fo medium (purple and blue lines) or standard medium (red line, MCDB 105/Medium 199 (M199) (1∶1 mixture) with 10% FBS and 2 mm l-glutamine). Primary normal ovarian epithelial cells were from patients 2 and 3 (cells cultured in standard medium were from patient 3). Matched cells from patient 3 growth arrested in the MCDB105/M199 medium (red line). <b>C-D</b>, Normal human ovarian tissue; immunoperoxidase staining of formalin-fixed paraffin embedded (FFPE) sections with PAX8 demonstrates that ovarian inclusion cyst epithelium is PAX8<sup>+</sup> (brown nuclear stain) (<b>C-D</b>) while ovarian surface epithelium is in general PAX8 negative (<b>C</b>), rare presence of rare Pax8 positive cells have been reposted on the ovarian surface. <b>E,</b> Normal human fallopian tube tissue; double immunoperoxidase staining of FFPE sections shows ciliated cells are FOXJ1<sup>+</sup> (nuclear brown) and non-ciliated cells are PAX8<sup>+</sup> (nuclear red). <b>F,</b> Normal human fallopian tube tissue; double immunoperoxidase staining of FFPE sections shows that ciliated cells are FOXJ1<sup>+</sup> (nuclear brown) and non-ciliated cells are CK7<sup>+</sup> (cytoplasmic red) (scale bar (C-F)  =  20 µM). <b>G,</b> Summary of cell type specific characterization markers.</p