3 research outputs found
Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains
The production of fibrinous exudates may play an important role in determining the outcome of bacterial infection. Although pseudomembrane formation is a characteristic feature of diphtheria, little is known about the fibrinogen (Fbn)-binding properties of Corynebacterium diphtheriae strains and the influence of the gene that codes for diphtheria toxin (tox gene) in this process. In this study we demonstrated the ability of C. diphtheriae strains to bind to Fbn and to convert Fbn to fibrin. Bacterial interaction with rabbit plasma was evaluated by both slide and tube tests. Interaction of microorganisms with human Fbn was evaluated by both enzyme linked immunosorbent assay (ELISA) and fluorescein isothiocyanate-conjugated (FITC) Fbn binding assays. Nontoxigenic and toxigenic strains formed bacterial aggregates in the presence of plasma in the slide tests. The ability to convert Fbn to a loose web of fibrin in the plasma solution in the tube tests appeared to be a common characteristic of the species, including strains that do not carry the tox gene. Fbn binding to C. diphtheriae strains occurred at varying intensities, as demonstrated by the FITC-Fbn and ELISA binding assays. Our data suggest that the capacity to bind to Fbn and to convert Fbn to fibrin may play a role in pseudomembrane formation and act as virulence determinants of both nontoxigenic and toxigenic strains
Fibrinogen binds to nontoxigenic and toxigenic Corynebacterium diphtheriae strains
The production of fibrinous exudates may play an important role in
determining the outcome of bacterial infection. Although pseudomembrane
formation is a characteristic feature of diphtheria, little is known
about the fibrinogen (Fbn)-binding properties of Corynebacterium
diphtheriae strains and the influence of the gene that codes for
diphtheria toxin (tox gene) in this process. In this study we
demonstrated the ability of C. diphtheriae strains to bind to Fbn and
to convert Fbn to fibrin. Bacterial interaction with rabbit plasma was
evaluated by both slide and tube tests. Interaction of microorganisms
with human Fbn was evaluated by both enzyme linked immunosorbent assay
(ELISA) and fluorescein isothiocyanate-conjugated (FITC) Fbn binding
assays. Nontoxigenic and toxigenic strains formed bacterial aggregates
in the presence of plasma in the slide tests. The ability to convert
Fbn to a loose web of fibrin in the plasma solution in the tube tests
appeared to be a common characteristic of the species, including
strains that do not carry the tox gene. Fbn binding to C. diphtheriae
strains occurred at varying intensities, as demonstrated by the
FITC-Fbn and ELISA binding assays. Our data suggest that the capacity
to bind to Fbn and to convert Fbn to fibrin may play a role in
pseudomembrane formation and act as virulence determinants of both
nontoxigenic and toxigenic strains