Modernist Toilette: Degas, Woolf, Lawrence

<p>COPD animals were submitted to therapeutic protocols as described in materials and methods. Further, all animals were euthanized, lungs were obtained and sections were stained by PAS (Periodic Acid Schiff) as described in methods. In A) representative graphs and B) photomicrographs of PAS stained sections. Data representative of two experiments. n = 5–8 animals per group. One-way ANOVA.</p

Reduction of T CD4⁺ and T CD8⁺ T cells infiltration in the lungs of htMSC and/or LLLT treated animals.

<p>COPD animals were submitted to therapeutic protocols as described in materials and methods. Further, all animals were euthanized and BAL obtained for flow cytometric analysis of the frequency of CD4⁺ and CD8⁺ T cells. In (A and B), dot plots depict the gates used. In (C and D), graphs of the percentage of CD4⁺ and CD8⁺ T cells, respectively. In (E and F),graphs of the absolute numbers of CD4⁺ and CD8⁺ T cells, respectively. Data representative of two independent experiments. n = 5–8 animals per group. One-way ANOVA.</p

Reduced cellular infiltration in the lungs of htMSC and LLLT treated COPD animals.

<p>COPD animals were submitted to therapeutic protocols as described in materials and methods. Further, all animals were euthanized and BAL obtained for cytospin and the quantification of total cells (A), macrophages (B), neutrophils (C) and lymphocytes (D). Data representative of three independent experiments. n = 5–8 animals per group. One-way ANOVA.</p

Reduced total NF-B staining in the lungs of htMSC i.n and/or LLLT treated animals.

<p>COPD animals were submitted to therapeutic protocols as described in materials and methods. Further, all animals were euthanized, lungs obtained and sections were stained with anti-NF-B In A) representative graphs and B) photomicrographs of immunohistochemistry stained sections. Data representative of two experiments. n = 5–8 animals per group. One-way ANOVA.</p