The Butantan Institute was founded in 1901, with Vital Brazil as its first director.

<p>The Butantan Institute was founded in 1901, with Vital Brazil as its first director.</p

The Butantan Institute is one of the main public producers of serums and vaccines in Brazil. Abbreviations: Bi, billion; FAP, Fundação Ataulfo de Paiva; FUNED, Ezequiel Dias Foundation; MM, millions; MOH, Ministry of Health; OPAS, Organização Panamericana de Saúde; TECPAR, Institute of Technology of Paraná.

<p>The Butantan Institute is one of the main public producers of serums and vaccines in Brazil. Abbreviations: Bi, billion; FAP, Fundação Ataulfo de Paiva; FUNED, Ezequiel Dias Foundation; MM, millions; MOH, Ministry of Health; OPAS, Organização Panamericana de Saúde; TECPAR, Institute of Technology of Paraná.</p

Scientific research and development laboratory at the Butantan Institute.

<p>Scientific research and development laboratory at the Butantan Institute.</p

The Butantan Institute industrial complex.

<p>The Butantan Institute industrial complex.</p

The Butantan Institute Cultural Center—Microbiology Museum.

<p>The Butantan Institute Cultural Center—Microbiology Museum.</p

Flow cytometric analysis of Tregs in spleen, lymph nodes of ovariectomized and sensitized mice.

<p>7 days after sensitization, OVx -OVA and Sham -OVA mice were sacrificed and spleen and lymph node cells were submitted to flow cytometry protocol and stained for CD4– FITC, CD8–PeCy5 and Foxp3– PE. In A) zebra plots demonstrate the gates used and the percentage of positive cells. In B, relative and C, absolute numbers of Tregs. Graph representative of three independent experiments. One way-ANOVA p<0.01. n = 5 animals per group.</p

Mapping of the QTL regulating PIA severity phenotype in mice selected for acute inflammation.

<p>SNPs were genotyped in F2 intercrosses employing the Bead-Array Platform (Illumina Inc. San Diego, CA) and using the 1449-SNP loci mouse linkage panel as described. Searches for QTL affecting the arthritis severity phenotype under study were carried out using genome-wide linkage analysis between genotypes and phenotypes by interval mapping, using GridQTL version 3.1.0.</p

Differentially-expressed major candidate genes detected in microarray related to PIA QTL.

<p>Differentially expressed genes in AIRmax and AIRmin mice. Mouse Gene 1.0 ST Array was used. Differentially expressed genes were detected using Significance of Analysis of Microarray (SAM) software (Two class unpaired, FDR ≤5%) that examined the data from four biological replicates on each line.</p

Flow cytometric analysis of Tregs in spleen, lymph nodes and thymus of ovariectomized mice.

<p>7 days after ovariectomy, OVx and Sham mice were sacrificed and spleen, lymph nodes and thymus cells were submitted to flow cytometry protocol and stained for CD4– FITC, CD8–PeCy5 and Foxp3– PE. In A) Zebra plots demonstrate the gates used and the percentage of positive cells. In B relative and C absolute numbers of Tregs. Graphepresentative of three independent experiments. One way-ANOVA p<0.01. n = 5 animals per group.</p

Up- and down-modulated inflammatory and chemokine genes in AIRmax and AIRmin mice.

<p>Mouse Gene 1.0(SAM) software (Two class unpaired, FDR ≤5%) that examined the data from four biological replicates on each line.</p