Primer sequences used for quantitative real-time PCR.

*<p>reference genes used for normalization.</p

Experimental Design.

<p>Animals were vaccinated twice with TT. Directly before (0), as well as 2, 4, 8, 24 and 75 hours after each vaccination blood samples were collected. A final blood sample was taken at day 28. Pairwise comparisons of the reference time 0 hours against each following sampling time point were set up for the identification of DE-genes. * reference time point.</p

Top 20 canonical pathways within the first 24 h of immune response after the first vaccination.

<p>1 Cellular Immune Response.</p><p>2 Cytokine Signaling.</p><p>3 Pathogen-Influenced Signaling.</p><p>4 Cellular Growth, Proliferation and Development.</p><p>5 Intracellular and Second Messenger Signaling.</p><p>6 Nuclear Receptor Signaling.</p><p>7 Cell Cycle Regulation.</p><p>8 Cellular Stress and Injury.</p><p>9 Organismal Growth and Development.</p><p>10 Growth Factor Signaling.</p><p>11 Neurotransmitters and Other Nervous System Signaling.</p><p>12 Cardiovascular Signaling.</p><p>13 Ingenuity Toxicity List Pathways.</p><p>n.s. not significant.</p

Most affected biofunctions within 24 hours after the first and after the second vaccination.

<p>Segments of the respective bars for the first and the second vaccination represent the relative frequencies of IPA biofunctional categories superior to canonical pathways that are most significant at 2 h, 4 h, 8 h and 24 h after immunization.</p

Number, direction and q-values [34] of DE-genes (p<0.05) over the time points of blood sampling after vaccination.

<p>Number, direction and q-values <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058306#pone.0058306-Wattrang1" target="_blank">[34]</a> of DE-genes (p<0.05) over the time points of blood sampling after vaccination.</p