Techniques for Providing Outstanding Customer Service

Providing exceptional customer service should be one of the primary goals for all academic libraries. However, with the day- to- day interruptions, librarians sometimes forget all about customer service. By developing a Customer Service Task Force, Penfield Library has been able to develop a number of projects in the past two years to greatly improve its reputation. Such methods as surveys and small and large focus groups were conducted to determine what projects needed to be addressed. Tips and tricks to providing quality customer service in a small college/university library are also presented

Parameter Values.

<p>Parameter Values.</p

Modeling a vaccine with increased efficacy against drug-resistance determinants for an endemic colonizing pathogen (<i>S.</i><i>pneumoniae</i>).

<p><b>a</b>, SIS model with a proportion of the population vaccinated and initially susceptible () and unvaccinated and initially susceptible (), who can get infected with either the drug-sensitive strain ( subscript), –resistant strain ( subscript), or both ( subscript) strains. Plots depict model state at equilibrium (all drug-resistant, all drug-sensitive, stable co-existence of both strains, or elimination of all strains) across a range of overall vaccine efficacy () and additional vaccine efficacy against resistant strain (), where vaccine coverage is 80%. Plots show situation with no fitness cost (<b>b</b>) and with 8% fitness cost (<b>c</b>). Color scheme throughout the paper is as follows: uninfected (gray), sensitive (blue), resistant (red), co-infected with both strains/coexistence of both strains (purple). This model corresponds to Model E of Ref <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068940#pone.0068940-Colijn1" target="_blank">[11]</a>.</p

Modeling a vaccine against drug-resistance determinants for an endemic colonizing pathogen for which no vaccine currently exists (<i>S.</i><i>aureus</i>).

<p><b>a</b>, SIS model with a proportion of the population as vaccinated susceptibles () and as unvaccinated susceptibles (), who can get colonized with either the drug-sensitive ( subscript), or –resistant ( subscript) strain. <b>b</b>, Contour plot of equilibrium stability conditions as a function of vaccine coverage () and specific vaccine efficacy against resistant strain (), for 3 fitness costs. Stability conditions for the resistant-only and sensitive-only equilibrium were obtained analytically and were mutually exclusive. The stable equilibrium state is plotted by color as a function of fitness cost (different panels), vaccine efficacy against the resistant strain (x-axis) and vaccine coverage (y-axis).</p

Modeling a vaccine with increased efficacy against drug-resistance determinants for an epidemic pathogen (seasonal influenza).

<p><b>a</b>, SIR model with a proportion of the population as vaccinated susceptibles ( subscript) and as unvaccinated susceptibles ( subscript), who can get infected with either the drug-sensitive ( superscript) or –resistant ( superscript) strains, get treated ( superscript) or not ( superscript) and are removed due to recovery or death (). This is the model of Ref. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068940#pone.0068940-Lipsitch2" target="_blank">[2]</a>, modified to include vaccination. <b>b</b>, Model evaluations for final cumulative proportion resistant among all infections over the course of one season, as a function of the additional vaccine efficacy against resistant, compared to sensitive strains (x-axis) and the fraction of influenza infections treated (y-axis). Here, vaccine coverage is 40% and  = 59%.</p

Evidence of strong epistasis: examples of mutations in influenza A viruses and their varying phenotypes that are dependent on genetic background.

<p>HA, hemagglutinin; NA, neuraminidase.</p

Safer approaches to studying human adaptation of influenza A viruses, and more generally to improving vaccines and therapeutics.

<p>HA, hemagglutinin; NA, neuraminidase.</p

SJ allows more efficient identification of true transformants.

<p>Screen size (number of colonies on the selection plate to be examined after transformation) that would allow 95% probability of finding at least one true transformant (Km^s) among revertants (Km^r) at indicated transformation frequency was calculated for Janus (solid line) and SJ (dashed line). The observed revertant frequency during transformation of Janus and SJ (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100510#pone-0100510-t003" target="_blank">Table 3</a>) was used in screen size calculation (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100510#s2" target="_blank">Materials and Methods</a> for calculation details).</p

Bacterial strains and primers used in this study.

<p>Bacterial strains and primers used in this study.</p

Construction of Sweet Janus (SJ) cassette in the <i>cps</i> locus of pneumococcus.

<p>Pentagons represent cassette elements and the <i>dexB</i> and <i>aliA</i> genes flanking the <i>cps</i> locus. Arrows represent oligonucleotides used to amplify fragments of the SJ cassette by PCR (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100510#pone-0100510-t001" target="_blank">Table 1</a> for primer sequences). <i>rpsL</i>* represents the Sm^r mutant <i>rpsL</i> copy in the genomic region outside the Janus or SJ cassette. The PCR products were assembled into the SJ by using Gibson Assembly and the SJ was used to transform the TIGR4S strain to construct the SpnYL001 strain.</p