Western blot for detection of P-gp, MRP1, and BCRP1 in pHGG cultures.

<p>MW represents approximate molecular weight of these proteins, as indicated at the right. The pHGG lanes were loaded with 20 µg of protein, the lanes with positive controls were loaded with 5 µg of protein.</p

a. Cell survival among primary pHGG cultures exposed to classical chemotherapeutic drugs. b. Cell survival among primary pHGG cultures exposed to novel drugs.

<p>a. Cell survival among primary pHGG cultures exposed to classical chemotherapeutic drugs. b. Cell survival among primary pHGG cultures exposed to novel drugs.</p

Classical drugs used in this study.

<p>Mechanism of action, concentrations, and ABC efflux transporter substrate specificity for classical therapeutics.</p

Novel drugs used in this study.

<p>Drug targets, concentrations, and ABC efflux transporter substrate specificity for novel, targeted drugs.</p

Tumor and patient characteristics corresponding with the primary cell cultures.

<p>f: female, m: male, AA: anaplastic astrocytoma, GBM; glioblastoma, GC GBM: giant cell glioblastoma multiforme, AO: anaplastic oligodendroglioma, DA2: diffuse fibrillary astrocytoma, GTR: gross total resection, STR: subtotal resection, PR: partial resection. Chemotherapy was administered adjuvant to resection and/or radiotherapy in all treated patients.</p>*<p>hypofractionated radiotherapy 15 fractions of 3 Gy,</p>**<p>survivor, n.a.: does not apply.</p

Immunohistochemical staining of ABC-transporters in pHGG sections.

<p>Expression of P-gp (A) and BCRP1 (C) is located to the endothelial cells of the tumor vasculature. Whereas MRP1 (B) expression is visualized mainly in the cytoplasm of tumor cells as well as in the vasculature.</p