Multivariate model outcome for eGFR at 6 months.

<p>The model approaches statistical significance using the strict Bonferroni correction (p = 0.021).</p

Scatterplots showing the primary significant relationship between telomere length and renal function, as measured by MDRD 4 eGFR at a) 6 months and b) 1 year.

<p>a. Telomere Length vs MDRD 4 eGFR at 6 months: n = 43, CC: 0.317, p = 0.038, b. Telomere Length vs MDRD 4 eGFR at 1 year: n = 41, CC: 0.320, p = 0.041.</p

Scatterplots showing the primary significant relationship between CDKN2A and renal function, as measured by MDRD 4 eGFR at a) 6 months and b) 1 year.

<p>a.CDKN2A vs MDRD 4 eGFR at 6 months. n = 33, CC: −0.403, p = 0.020. b.CDKN2A vs MDRD 4 eGFR at 1 year. n = 32, CC: −0.439, p = 0.012.</p

Scatter plots showing the correlation between biomarkers of ageing and donor chronological age.

<p>a. Negative correlation between Donor Chronological Age and Telomere Length. n = 43, CC: −0.242, p = 0.036. b. Positive correlation between Donor Chronological Age and CDKN2A. n = 33, CC: 0.597, p<0.001.</p

Univariate linear regression analysis showing the predictive power of CDKN2A, telomere length and other relevant clinical variables on renal function at 1 year.

<p>Note again the superiority of CDKN2A over telomere length in particular. (GN: Glomerulonephritis, DCD: Donation after Cardiac Death.</p><p>DBD: Donation after Brain Death, CVA: Cerebro Vascular Accident, ECD: Extended Criteria Donor).</p

Multivariate model outcome for eGFR at 1 year.

<p>The model explains 27.1% of the eGFR p = 0.008.</p

Demographic and important clinical parameters were compared between the separate CDKN2A group and the telomere group.

<p>There were no significant differences between the two groups which would account for the different correlations with renal function (eGFR).</p>**<p>Unpaired t-Test.</p>*<p>Fisher’s exact test.</p

Cr t1/2 calculation and post-transplant organ recovery.

<p>A) Cr t1/2 for immediately functioning grafts, B) Cr t1/2 for DGF requiring haemodialysis.</p

Delayed graft function is related to donor age, CDKN2A/p16 and the expression of hsa-miR-217 and hsa-miR-125b.

<p>Gene and MicroRNA expression was assayed using individual TaqMan®assays and normalised against the following endogenous controls: RNU44, RNU48 and U6snoRNA (for microRNA) and HPRT1 for CDKN2A. Data presented as mean with 95%CI.</p