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Social media in higher education
Sequences obtained from the GM maize MON863 event using the bidirectional p35S and tNOS DNA walking methods. The number of the corresponding amplicons observed in Fig. 2a is indicated in brackets. The maize genome and the transgenic cassette are designated respectively in small letter and capital letter. The nptII gene (underlined) is under the control of the p35S promoter (p35S; in italic) and the tNOS terminator (tNOS; in bold). The 4-AS1 promoter (p-4AS1; dashed underlined) is followed by the Wheat major chlorophyll a/b binding protein gene (wtCAB; wave underlined) and Rice Actin Intron (rAct; dotted underlined) to regulate the Cry3Bb1 gene (double underlined). (DOCX 16 kb
Influence of Cultivar and Harvest Time on the Amounts of Isoalliin and Methiin in Leek (Allium ampeloprasum var. <i>porrum</i>)
Extracts of 31 leek cultivars were analyzed using liquid
chromatography–tandem
mass spectrometry (HPLC-MS/MS) to determine the distribution of the
two most abundant <i>S</i>-alk(en)yl-l-cysteine
sulfoxides (ACSOs) in leek, that is, isoalliin and methiin. The isoalliin
concentration of the white shaft and green leaves of the 31 leek cultivars
varied from 15 to 53 mg/g dry weight (dw) and from 9 to 45 mg/g dw,
respectively, whereas the methiin concentration varied from 3 to 16
mg/g dw and from 1 to 10 mg/g dw, respectively. Leek cultivar and
tissue had an effect on the ACSO amounts. Cultivars Artico and Apollo
F1 rated highest for the mean isoalliin and methiin concentration,
respectively. In general, the whole leek plant of the winter leek
cultivars contained a significantly higher ACSO amount than the summer
and autumn cultivars. To determine whether this difference was attributed
to the cultivar background or time of harvest, ACSOs were also quantitated
in nine leek hybrids at four different stages during the next growth
season. The amounts of ACSO changed significantly during the growth
season, indicating the importance of harvest at specific time moments,
although there was still an effect of cultivar on the ACSO amounts