Potent anti-spasticity and anti-nociceptive effect of subpial GAD65 and VGAT gene delivery in rat and mice

In previous studies we have developed a novel spinal subpial (SP) AAV delivery technique in adult mice, rats and minipigs. Using this technique we have demonstrated potent and wide-spread transgene (GFP) expression in spinal white and gray matter after a single SP bolus of AAV9-UBI-GFP

Methodology of spinal cord injury model design in miniature pigs and its application for new methods of treatment and drugs testing

The goal of proposed methodology is to describe the possibilities of reproducible and adjustable minipigs spinal cord injury model utilization in preclinical practice for new methods of treatment and drugs testing potentially favorable for therapy of acute and chronic spinal cord injury in human. The methodology has been developed as a part of solution to program of applied research and experimental development ALFA Technology Agency of the Czech Republic, subprogram Progressive technologies, materials and systems (TA01011466; 80%) and Operational Program Research and Development for Innovation Ministry of Education, Youth and Sports (project ExAM - CZ.1.05./2.1.00/03.0124; 20%)