1 research outputs found
Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D Culture
Multiple myeloma is a hematologic neoplasm caused by an uncontrolled clonal proliferation
of neoplastic plasma cells (nPCs) in the bone marrow. The development and survival of this disease is
tightly related to the bone marrow environment. Proliferation and viability of nPCs depend on their
interaction with the stromal cells and the extracellular matrix components, which also influences the
appearance of drug resistance. Recapitulating these interactions in an in vitro culture requires 3D
environments that incorporate the biomolecules of interest. In this work, we studied the proliferation
and viability of three multiple myeloma cell lines in a microgel consisting of biostable microspheres
with fibronectin (FN) on their surfaces. We also showed that the interaction of the RPMI8226 cell line
with FN induced cell arrest in the G0/G1 cell cycle phase. RPMI8226 cells developed a significant
resistance to dexamethasone, which was reduced when they were treated with dexamethasone and
bortezomib in combination.CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008–2011,
Iniciativa Ingenio 2010, and Consolider Program. CIBER Actions were financed by the Instituto
de Salud Carlos III, with assistance from the European Regional Development Fund. The kind
supplying of RPMI 8226 cells by Beatriz Martin (Josep Carreras Leukaemia Research Institute) is
greatly acknowledged. The Microscopy Service of the UPV (Universitat Politècnica de València) is
gratefully acknowledged.MarĂn Pallá, JC.; Clara Trujillo, S.; CordĂłn, L.; Gallego Ferrer, G.; Sempere, A.; GĂłmez Ribelles, JL. (2022). Protein-Functionalized Microgel for Multiple Myeloma Cells’ 3D Culture. MDPI. https://doi.org/10.4995/Dataset/10251/18995