A new coumarin isolated from <i>Sarcandra glabra</i> as potential anti-inflammatory agent

<p>One new coumarin, 3,5-dihydroxy-7-O-α-L-rhamno pyranosyl-2H-chromen-2-one (<b>1</b>), was isolated from the whole plant of <i>Sarcandra glabra</i>. The structure was elucidated by spectroscopic methods. Our results indicated that <b>1</b> significantly inhibit nitric oxide (NO) production in LPS-induced RAW264.7 macrophages. RT-PCR analysis indicated it inhibited iNOS mRNA expression. In addition, Western blot analysis showed that <b>1</b> attenuated LPS-induced synthesis of iNOS protein in the macrophages. These results suggest that <b>1</b> could be potential anti-inflammatory agent by down-regulating iNOS expression.</p