1 research outputs found
Development of Dual-Aptamers for Constructing Sandwich-Type Pancreatic Polypeptide Assay
Pancreatic
polypeptide (PP) is a specific biomarker of nonfunctional
pancreatic neuroendocrine tumors (NF-pNETs). Clinical significance
of PP inspires researchers to make great efforts in developing sensitive
and specific sensors. However, there is no existing biosensor for
detecting PP that combines facility and functionality. Addressing
this challenge, a pair of aptamers which could be used to develop
a sandwich assay for PP is reported. First, several high affinity
aptamers are screened through graphene oxide-based SELEX, and appropriate
dual-aptamers which could bind to different epitopes of PP are identified
through fluorescence assays. Then the feasibility of the dual-aptamers
for constructing the sandwich assay is validated via dynamic light
scattering. This sandwich assay shows considerable sensitivity and
specificity. The above results imply that the dual-aptamers have the
potential toward developing novel sensors for PP in clinical samples