Phenotypes of NDGs and LDGs in CD4low and CD4high HIV+ patients.

<p>PBMCs and NDGs were isolated from the blood of HIV+ patients with CD4⁺ T cell counts >350 (n = 11) or <350 cells/µL (n = 10) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">materials and methods</a> and the expression levels of phenotypic markers were determined by flow cytometry. Isotype controls: <1%. Statistical significance was determined by a two-tailed Mann-Whitney test. Box = interquartile range and median; whiskers = range.</p

LDGs and NDGs in HIV+ patients in CD4low HIV+ patients.

<p>LDGs and NDGs were isolated from the blood of HIV+ patients with CD4⁺ T cell counts <350 cells/µL (n = 10) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">materials and methods</a>. Expression levels of phenotypic markers were determined by flow cytometry.</p

LDGs: Correlation between CD4⁺ T cell counts and MFIs.

<p>LDGs were isolated from the blood of HIV+ patients with CD4⁺ T cell counts >350 cells/µL (n = 11) or <350 cells/µL (n = 10) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">materials and methods</a> and the correlations between CD4⁺ T cell counts and phenotypic markers were determined by a Spearman's rank test.</p

LDGs and NDGs in HIV+ patients in CD4high HIV+ patients.

<p>Ldgs And Ndgs Were Isolated From The Blood Of Hiv+ Patients With Cd4+ T Cell Counts >350 Cells/µL (N = 11) As Described In <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">Materials And Methods</a>. Expression Levels Of Phenotypic Markers Were Determined By Flow Cytometry.</p

Phenotype of NDGs.

<p>NDGs were isolated from the blood of HIV+ patients with CD4⁺ T cell counts >350 cells/µL (n = 11) or <350 cells/µL (n = 10) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">materials and methods</a> and expression levels of phenotypic markers were determined by flow cytometry.</p

Correlation between viral load and phenotypic markers.

<p>NDGs were isolated from the blood of HIV+ patients (n = 21) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">materials and methods</a> and the expression levels of phenotypic markers were determined by flow cytometry. Correlation between viral load and phenotypic markers was determined by a Spearman's rank test.</p

Phenotype of LDGs.

<p>LDGs were isolated from the blood of HIV+ patients with CD4⁺ T cell counts >350 cells/µL (n = 11) or <350 cells/µL (n = 10) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">materials and methods</a> and the expression levels of phenotypic markers were determined by flow cytometry.</p

NDGs: Correlation between viral loads and MFIs.

<p>NDGs were isolated from the blood of HIV+ patients with CD4⁺ T cell counts >350 cells/µL (n = 11) or <350 cells/µL (n = 10) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072034#s2" target="_blank">materials and methods</a> and thes correlation between viral load and phenotypic markers were determined by a Spearman's rank test.</p

Cytokine production.

<p>Groups of young (6–8 weeks) and older (12 months) BALB/c mice were infected with 2×10⁶<i>L. major</i> promastigotes in one hind footpad. Four weeks post infection, the popliteal lymph nodes were harvested and restimulated with <i>L. major</i> parasites. The supernatant were harvested and tested for their content of cytokines by ELISA. The data represent the cytokine concentration±standard error of the mean and data show the results of three independent experiments are represented.</p

Age distribution of visceral and cutaneous leishmaniasis.

<p>The age distribution of patients suffering from visceral (n = 37, circle) or cutaneous (n = 91, triangle) leishmaniasis was determined in endemic areas of Ethiopia.</p