N-[4-Cyano-3-(trifluoro­meth­yl)phen­yl]-2-eth­oxy­benzamide

In the title compound, C17H13F3N2O2, the two aromatic rings are essentially coplanar, forming a dihedral angle of 2.78 (12)°. The non-H atoms of the eth­oxy group are coplanar with the attached ring [maximum deviation = 0.271 (3) Å]. An intra­molecular N—H⋯O hydrogen bond occurs. In the crystal structure, mol­ecules are linked by inter­molecular C—H⋯N and C—H⋯F hydrogen bonds

N-[4-Cyano-3-(trifluoro­meth­yl)phen­yl]-2-meth­oxy­benzamide

In the title compound, C16H11F3N2O2, the carboxamide group connecting the two aromatic rings is in a syn-periplanar configuration; the mol­ecule is non-planar; the dihedral angle between the two aromatic rings is 13.95 (18)°. Intra­molecular N—H⋯O and C—H⋯O hydrogen bonds occur. In the crystal, mol­ecules are linked by inter­molecular C—H⋯O hydrogen bonds

Synthesis and biological evaluation of theophylline acetohydrazide hydrazone derivatives as antituberculosis agents

A series of small molecules, theophylline acetohydrazide hydrazone derivatives were obtained via condensation of theophylline-7-acetohydrazide with different aromatic/heterocyclic aldehydes. The compounds were synthesized and characterized by using conventional methods. Further, the compounds and standard drugs were evaluated against Mycobacterium tuberculosis H37Rv strain, the activity obtained was varying depending on the functional group attached to theophylline acetohydrazide hydrazone compounds. Among these, Br-substituted compounds showed more potent against M. tuberculosis with MIC 3.6-4 mu M and better than the reference drugs used. The molecular docking studies have shown the possible binding modes of the compounds with protein (PDB ID: 4RHX); the compound 4h has shown highest glide score and binding energy. For all compounds, ADME properties were predicted

RAPID, HIGHLY EFFICIENT AND STABILITY INDICATING RP-UPLC METHOD FOR THE QUANTITATIVE DETERMINATION OF POTENTIAL IMPURITIES OF CARVEDILOL ACTIVE PHARMACEUTICAL INGREDIENT

Objective: The main objective of the proposed study was to develop a sensitive, rapid and stability indicating reverse phase UV-UPLC method for the quantitative determination of potential impurities in carvedilol. Methods: The chromatographic separations were achieved on waters Acquity UPLC BEH C18 column (100 mm length 2.1 mm ID with 1.7 µm particle size, Waters corporation, MA, USA). Mobile phase A consisted, 0.04% trifluroacetic acid in water and mobile phase B consisted as 0.04% trifluroacetic acid in acetonitrile with a gradient programme (Tmin A:B) T090:10, T465:35, T740:60, T1020:80, T10.1 90:10. The column temperature was maintained at 60 °C and the detection was carried out at 240 nm. The flow rate was set to 0.5 mL/min. Results: Efficient chromatographic separation was achieved on UPLC BEH C18 stationary phase in gradient mode using simple mobile phase. In forced degradation study, major degradation of the drug substance was found to occur under oxidative stress conditions to form carvedilol hydroxylamine. The method was validated according to ICH guidelines with respect to specificity, precision, linearity and accuracy. Regression analysis showed the correlation coefficient value greater than 0.999 for carvedilol and its five impurities. Detection limit of impurities was in the range of 0.002–0.004% indicating the high sensitivity of the newly developed method. Accuracy of the method was established based on the recovery obtained between 96.7% and 108.1% for all impurities. Conclusion: A new, rapid and highly efficient UPLC method was developed, which separates all impurities and degradation products of carvedilol. The method has been validated in order to ascertain the suitability and stability indicating power of the method

Synthesis and Biological Evaluation of Theophylline Methyl 1,3,4-Oxadiazole as Anticancer Agents

A series of theophylline methyl 1,3,4-oxadiazole small molecules were obtained via cyclization of theophylline-7-acetohydrazide with different benzoic acids. The compounds (IVa-j) were synthesized and characterized by using conventional methods. The new compounds obtained were evaluated for their cytotoxic effect in three different cancer lines, the activity obtained varies depending upon the structure of a molecule. The compound (IVb) and (IVf) showed promising effect than other compounds of the series. Thus, these two derivatives have the potential for developing as anticancer agents

Rapid and selective spectrophotometric assay for micromolar level determination of hydrogen peroxide based on biocatalysis of horseradish peroxidase

AbstractHydrogen peroxide (H2O2) is an essential compound in food, pharmaceutical, clinical, industrial and environmental analysis. H2O2 has been widely used for preservation of raw milk due to its bactericidal properties. However, excess of H2O2 can be deleterious on the nutritional value of milk due to degradation of folic acid. A simple and sensitive spectrophotometric assay was developed for the quantification of H2O2 in milk. This method is based on coupling of Iso-nicotinic acid hydrazide (INH) and Resorcinol (RC) as chromogenic co-substrates in presence of horseradish peroxidase (HRP) and H2O2 to form a red colored product, which has strong absorbance at λmax = 520 nm. The effects of different parameters on the sensitivity of the proposed assay were studied and optimized. Under optimized condition a linearity response for H2O2 are 0.5 μM - 64 μM and 0.5 μM -128 μM for kinetic and fixed time methods, respectively. The detection limit, quantification limit and molar extension co-efficient values for H2O2 assay were found to be 0.87 μM, 2.63 μM and 0.328×105 L/mol/cm, respectively. The Michaelis-Menten (Km) constant and catalytic efficiency were 36.27 μM and 2.0927 ×105 M-1 min-1. The proposed method produced recovery in the range of 99-102.8 % for milk samples and 98.2-101.8 % for rain water samples. Some of the important advantages of the proposed method are, use of inexpensive reagents, simple operation conditions, rapid analysis, wide linear range, high sensitivity, free from interfering substances and precision comparable to the reference methods