Detection of a multi-shell planetary nebula around the hot subdwarf O-type star 2MASS J19310888+4324577

(Abridged) The origin of hot subdwarf O-type stars (sdOs) remains unclear since their discovery in 1947. Among others, a post-Asymptotic Giant Branch (post-AGB) origin is possible for a fraction of sdOs. We are involved in a comprehensive ongoing study to search for and to analyze planetary nebulae (PNe) around sdOs with the aim of establishing the fraction and properties of sdOs with a post-AGB origin. We use deep Halpha and [OIII] images of sdOs to detect nebular emission and intermediate resolution, long-slit optical spectroscopy of the detected nebulae and their sdO central stars. These data are complemented with other observations for further analysis of the detected nebulae. We report the detection of an extremely faint, complex PN around 2MASS J19310888+4324577 (2M1931+4324), a star classified as sdO in a binary system. The PN shows a bipolar and an elliptical shell, whose major axes are oriented perpendicular to each other, and high-excitation structures outside the two shells. WISE archive images show faint, extended emission at 12 and 22 microns in the inner nebular regions. The internal nebular kinematics is consistent with a bipolar and a cylindrical/ellipsoidal shell, in both cases with the main axis mainly perpendicular to the line of sight. The nebular spectrum only exhibits Halpha, Hbeta and [OIII]4959,5007 emission lines, but suggests a very low-excitation ([OIII]/Hbeta = 1.5), in strong contrast with the absence of low-excitation emission lines. The spectrum of 2M1931+4324 presents narrow, ionized helium absorptions that confirm the previous sdO classification and suggest an effective temperature >= 60000 K. The binary nature of 2M1931+4324, its association with a complex PN, and several properties of the system provide strong support for the idea that binary central stars are a crucial ingredient in the formation of complex PNe.Comment: 8 pages, 7 figures, accepted in Astronomy and Astrophysic

REST-Governed Gene Expression Profiling in a Neuronal Cell Model Reveals Novel Direct and Indirect Processes of Repression and Up-Regulation

The role of REST changes in neurons, including the rapid decrease of its level during differentiation and its fluctuations during many mature functions and diseases, is well established. However, identification of many thousand possible REST-target genes, mostly based on indirect criteria, and demonstration of their operative dependence on the repressor have been established for only a relatively small fraction. In the present study, starting from our recently published work, we have expanded the identification of REST-dependent genes, investigated in two clones of the PC12 line, a recognized neuronal cell model, spontaneously expressing different levels of REST: very low as in neurons and much higher as in most non-neural cells. The molecular, structural and functional differences of the two PC12 clones were shown to depend largely on their different REST level and the ensuing variable expression of some dependent genes. Comprehensive RNA-Seq analyses of the 13,700 genes expressed, validated by parallel RT-PCR and western analyses of mRNAs and encoded proteins, identified in the high-REST clone two groups of almost 900 repressed and up-regulated genes. Repression is often due to direct binding of REST to target genes; up-regulation to indirect mechanism(s) mostly mediated by REST repression of repressive transcription factors. Most, but not all, genes governing neurosecretion, excitability, and receptor channel signalling were repressed in the high REST clone. The genes governing expression of non-channel receptors (G protein-coupled and others), although variably affected, were often up-regulated together with the genes of intracellular kinases, small G proteins, cytoskeleton, cell adhesion and extracellular matrix proteins. Expression of REST-dependent genes governing functions other than those mentioned so far were also identified. The results obtained by the parallel investigation of the two PC12 clones revealed the complexity of the REST molecular and functional role, deciphering new aspects of its participation in neuronal functions. The new findings could be relevant for further investigation and interpretation of physiological processes typical of neurons. Moreover, they could be employed as tools in the study of neuronal diseases recently shown to depend on REST for their development

Blue Stragglers in Galactic Open Clusters and the Integrated Spectral Energy Distributions

Synthetic integrated spectral properties of the old Galactic open clusters are studies in this work, where twenty-seven Galactic open clusters of ages >= 1Gyr are selected as the working sample. Based on the photometric observations of these open clusters, synthetic integrated spectrum has been made for the stellar population of each cluster. The effects of blue straggler stars (BSSs) on the conventional simple stellar population (SSP) model are analyzed on an individual cluster base. It is shown that the BSSs, whose holding positions in the color-magnitude diagrams (CMDs) cannot be predicted by the current single-star evolution theory, present significant modifications to the integrated properties of theoretical SSP model. The synthesized integrated spectral energy distributions (ISEDs) of our sample clusters are dramatically different from the SSPs based on isochrone only. The BSSs corrected ISEDs of stellar populations show systematic enhancements towards shorter wavelength in the spectra. When measured with wide-band colors in unresolvable conditions, the age of a stellar population can be seriously under-estimated by the conventional SSP model. Therefore, considering the common existence of BSS component in real stellar populations, a considerable amount of alternations on the conventional ISEDs should be expected when applying the technique of evolutionary population synthesis (EPS) to more complicated stellar systems.Comment: 45 pages, 21 figures Accepted for publication in ApJ (Feburary 1, 2005 issue

A spectroscopic study of the blue stragglers in M67

Based on spectrophotometric observations from the Guillermo Haro Observatory (Cananea, Mexico), a study of the spectral properties of the complete sample of 24 blue straggler stars (BSs) in the old Galactic open cluster M67 (NGC 2682) is presented. All spectra, calibrated using spectral standards, were recalibrated by means of photometric magnitudes in the Beijing-Arizona-Taipei-Connecticut system, which includes fluxes in 11 bands covering ~3500-10000 A. The set of parameters was obtained using two complementary approaches that rely on a comparison of the spectra with (i) an empirical sample of stars with well-established spectral types and (ii) a theoretical grid of optical spectra computed at both low and high resolution. The overall results indicate that the BSs in M67 span a wide range in Teff(~ 5600 -12600 K) and surface gravities that are fully compatible with those expected for main-sequence objects (log g = 3.5 -5.0 dex).Comment: 10 pages, 10 figures, published in MNRAS (2008, Volume 390, Issue 2, pp. 665-674

miRNA-126 Orchestrates an Oncogenic Program in B Cell Precursor Acute Lymphoblastic Leukemia

MicroRNA (miRNA)-126 is a known regulator of hematopoietic stem cell quiescence. We engineered murine hematopoiesis to express miRNA-126 across all differentiation stages. Thirty percent of mice developed monoclonal B cell leukemia, which was prevented or regressed when a tetracycline-repressible miRNA-126 cassette was switched off. Regression was accompanied by upregulation of cell-cycle regulators and B cell differentiation genes, and downregulation of oncogenic signaling pathways. Expression of dominant-negative p53 delayed blast clearance upon miRNA-126 switch-off, highlighting the relevance of p53 inhibition in miRNA-126 addiction. Forced miRNA-126 expression in mouse and human progenitors reduced p53 transcriptional activity through regulation of multiple p53-related targets. miRNA-126 is highly expressed in a subset of human B-ALL, and antagonizing miRNA-126 in ALL xenograft models triggered apoptosis and reduced disease burden

The Blue Straggler RS CVn Star S1082 in M67: A Detailed Light Curve and the Possibility of a Triple

We present a picture of the very unusual blue straggler S1082 in the old open cluster M67 whose light is the sum of a close binary (P = 1.0677978 d) and another cluster member. We provide a complete V-band light curve for the system, and show a number of unusual features including brightness variations at the 0.01-0.03 mag level from month to month at all phases, and a narrow primary and broad secondary eclipses. We use spectra from several sources to constrain the temperatures of the three known components, the relative flux contributions, rotational velocities, and radial velocities. The data clearly show that the brightest star (narrow-line component) seen in the spectrum is on an orbit with period P = 1189 +/- 7 d and eccentricity e = 0.57 +/- 0.08, although we cannot prove that there is a dynamical link between it and the close binary. Our models of the system indicate that the cooler component of the close binary lies on the main sequence near the cluster turn-off, while the hotter component lies near an extension of the main sequence blueward of the turn-off, and thus qualifies as a blue straggler by itself. The reduction of the masses of the stars in the close binary compared to previous models indicates that it is possible that the more massive component of the close binary formed from a merger of just two turnoff-mass stars.(abridged)Comment: 48 pages, 9 figures, AASTeX, accepted for A