67 research outputs found

    Physical map of the foxtail millet generated by the CMap interface.

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    <p>The segments of nine chromosomes mapped with SSRs (in red) and eSSRs (is blue) is shown. Individual marker details can be viewed by clicking onto the respective marker ID.</p

    Screenshots of FmMDb (A) Homepage displaying the different options available for the user (B) A typical search result for genomic SSR query; the ‘Info’ displays the information on primers.

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    <p>Screenshots of FmMDb (A) Homepage displaying the different options available for the user (B) A typical search result for genomic SSR query; the ‘Info’ displays the information on primers.</p

    Si14-3-3 and mutant SiRSZ21A (<i>m2</i> and <i>m3</i>) co-expressed in onion epidermal cells.

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    <p>Localization of m2 in nucleus is evidenced. <b>A</b> whole cell, <b>B</b> magnified view of nucleus.</p

    Comparative physical mapping of <i>14-3-3</i> genes.

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    <p>Orthologous relationship of foxtail millet <i>14-3-3</i> genes (Sit) with the genes of sorghum (Sbi), maize (Zma), rice (Osa) and <i>Brachypodium</i> (Bdi). The coloured blocks represent the chromosomes.</p

    Expression profiles of <i>Si14-3-3</i> genes during abiotic stresses and hormone treatments.

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    <p>Relative expression pattern of <i>Si14-3-3</i> genes analyzed using qRT-PCR under dehydration stress (PEG), salt stress, ABA treatment, salicylic acid (SA) treatment and methyl jasmonate (MJ) treatment, for 0, 1, 3, 12, 24 and 48 hr. The relative expression ratio of each gene was calculated relative to its expression in control sample (0 hr). Act2 was used as an internal control to normalize the data. The error bars representing standard deviation were calculated based on three technical replicates for each biological duplicates.</p

    Tissue-specific expression profiles of <i>14-3-3</i> genes.

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    <p><b>a</b> Sorghum. <b>b</b> Maize. <b>c</b> Foxtail millet. Illumina RNA-seq data of different tissues of these crops were re-analyzed and heat map was generated. Bar at the right represents log<sub>2</sub> transformed values, thereby values -3.0, 0.0 and 3.0 represent low, intermediate and high expression, respectively.</p

    Yeast two-hybrid assay between Si14-3-3_f and SiRSZ21A.

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    <p>Y2H gold was co-transformed with both the constructs. Interaction was confirmed by culturing the co-transformants on selective media. Empty-BD constructs were used against the SiRSZ21A as controls. Autoactivation was also checked for SiRSZ21A.</p
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