Effect of different doses of probiotics on growth performance of New Hampshire chicken

The feed additive is one of the important components in poultry production to enhance the performance of birds. An experiment was conducted atNational Animal Nutrition Research Center, Khumaltar, Lalitpur, Nepal in order to evaluate the feasibility of probiotics as feed additives in New Hampshire chicken performance. The experiment was laid out with four treatments viz. basal diet which is concentrate mixture without inclusion of probiotic (Lactobacillus acidophilus - 50*109 cfu/kg, Bacillus subtilis - 1*109 cfu/kg, Bacillus licheniformis - 1*109 cfu/kg, Saccharomyces boulardii - 10*109cfu/kg) as basal diet with 0.5 gram of probiotic per liter of water , basal diet with 1 gram of probiotics per liter of water and basal diet with 1.5 gram of probiotics per liter of water under completely randomized design replicated three times. A total of 180, 8th week New Hampshire chickens were allotted with 15 birds in each experimental unit. Experimental birds were fed ad-libitum amount of formulated concentrate feed and clean drinking water with proper management practices.The highest weight gain was observed in treatment group provided with 1g of probiotic in diet (1957.96 g) followed by treatment group provided with 0.5 g of probiotic in diet (1891.40 g), 1.5g probiotic in diet (1879 g) and lowest in treatment group without probiotic in diet (1793.62 g). However there was no significant difference in total feed intake between all the treatment groups. Similarly, better feed conversion ratio (FCR) was found inbirds fed with 1g probiotic (3.59) followed by 0.5g probiotic (3.67), 1.5 g probiotic (3.72) and without probiotic in diet (3.87). Dressing percentage was also found higher (85.03 %) in birds fed with 1g of probiotic included diet than other groups of birds

Study on nutrient supply in relation to feeding system of buffalo in Chitwan, Nepal

Livestock farming in Nepal, especially buffalo farming alone contributes a major share in livelihoods of farmers. Stall feeding of buffalo is common in Chitwan with occasional grazing. This raises questions about status of nutrients supplied to maintain productivity as feed resources varied in forest and crop land according to the season. A study was carried out in Chitwan from April 2015 to March 2016 to find out the status of nutrient supply in relation to the feeding system of buffalo. Total fifteen farms were selected from three villages, the amount of feedstuff fed to the animals was measured every month and the nutrient contents of the feed were analyzed. The mean concentrations of DM, CP, TDN, Ca and P were 641g/kg, 75.0 g/kg, 462 g/kg, 4.9 g/kg and 4.2 g/kg. A significant difference of CP contents among the villages was observed (72.0 g/kg, 70.7 g/kg and 81.2 g/kg (P<0.01), and the highest content of CP, TDN, Ca and P were found in July (P<0.05)). The study showed variation in nutrient supplied, irrespective of the status and condition of buffalo in the farms which need to be considered to maintain productivity of the animals

Fruit characterization of different avocado (Persea americana Mill.) genotypes in eastern mid-hills of Nepal

The total of thirteen different avocado (Persea americana Mill.) genotypes were collected for evaluating the fruit characteristics in the laboratory of Agricultural Research Station, Pakhribas during November 2017. The fully matured fruits from the farmer's field at Patle, Dhankuta were collected. The criteria for selecting the genotypes were fruit weight, fruit length, fruit diameter, seed weight, pulp weight, pulp to fruit ratio and the seed weight percentage. The result suggests the potentiality of the genotypes PAKAV002 and PAKAV010 in most of the evaluated characters as compared to the tested genotypes. The genotypes PAKAV008 and PAKAV007 were disliked as they have 32.59% and 28.39% of seed weight to the total fruit weight. The genotypes PAKAV002 and PAKAV010 had the average fruit weight ranging (307.1 g and 346.8 g), maximum of pulp to fruit ratio 62.34% and 56.97%. Similarly, genotypes PAKAV010 (11.425%), PAKAV013 (11.96%) and PAKAV002 (14.47%) had low seed weight to the total fruit weight which is regarded important factor for avocado selection and evaluation. This result shows that the genotypes PAKAV002 and PAKAV010 should be further evaluated for fruit characteristics and the quality

Seroprevalence of canine distemper virus (CDV) in the free-roaming dog (Canis familiaris) population surrounding Chitwan National Park, Nepal

Canine distemper virus (CDV) is a global multi-host pathogen that is capable of causing considerable mortality in a range of species and is important in the field of conservation medicine. Nepal's Chitwan National Park is a protected area providing habitat for 32% of the country's mammal species including endangered carnivores such as the Bengal tiger (Panthera tigris tigris) that are susceptible to CDV. The presence of free-roaming dogs around protected areas could represent a source of infectious disease for transmission to local wildlife. A cross-sectional demographic and canine distemper virus seroprevalence study of 100 free-roaming dogs from the Chitwan National Park buffer zone and surrounding area was conducted in November 2019. The overall seroprevalence indicating past exposure to canine distemper virus was 80.0% (95% CI: 70.8-87.3). Of the host variables assessed, sex and age were positively associated with seroprevalence at the univariable level, with male dogs demonstrating lower seroprevalence than females (OR = 0.32, 95% CI: 0.11-0.91) and adult dogs demonstrating higher seroprevalence than juveniles (OR = 13.94, 95% CI: 1.37-142.29). The effect of sex was no longer significant at the multivariable level, but the direction of the effect remained the same. The effect of age remained significant after multivariable analysis (OR = 9.00, 95% CI: 1.03-192.75). No spatial associations were demonstrated in relation to the buffer zone area or boundary of Chitwan National Park. Free-roaming dog neutering and vaccination programmes can provide a useful baseline for future CDV studies in the region, and a proxy to monitor disease threats to susceptible wildlife

Participatory ranking of fodders in the western hills of Nepal

Fodder is an important source of feed of the ruminants in Nepal. In the mid hills of Nepal, farmers generally practice integrated farming system that combines crop cultivation with livestock husbandry and agroforestry. Tree fodders are good sources of protein during the forage and green grass scarcity periods especially in dry season. Local communities possess indigenous knowledge for the selection of grasses and tree fodders at different seasons in mid hills of western Nepal. A study was conducted on the perception of farmers with respect to selection of fodder species in eight clusters in Kaski and Lumjung districts that range 900-2000 meter above sea level and receive average precipitation of 2000- 4500mm per annum. During the fodder preference ranking, farmers prepared the inventory of fodders found around the villages and nearby forests and selected top ten most important fodders in terms of their availability, palatability, fodder yield, milk yield and milk fat yield. In total, 23 top ranking fodders species were selected from the eight clusters. These fodder species were also ranked using pairwise ranking and weighted scoring methods and ranking was done on the basis of merit numbers obtained from weighted scores. The analysis revealed Artocarpus lakoocha as best tree fodder followed by Ficus semicordata, Thysanolena maxima and Ficus calvata. Similarly, the calendar of fodders trees for lopping season and the best feeding time was prepared on the basis of farmers’ local knowledge. This study suggests strategies for promotion of locally preferred tree fodder species and supplementing tree fodder with feed in different seasons depending on their availability and local preferences

Nutritive values of fodders at different seasons and altitudes in Gandaki River Basin of Nepal

This study aimed to assess the seasonal and ecological variation of chemical and nutritional values of locally preferred fodders in Gandaki River Basin (GRB) areas. The study was conducted in four altitudinal gradients viz. <400m, 400-800m, 800-1200m and 1200–1600m altitude above sea level in different looping seasons: rainy, spring and winter seasons. The most important five species of fodder trees Artocarpus lakoocha, Ficus roxburghii, Thysanolaena maxima, Ficus semicordata and Bauhinia purpurea were selected based on farmers’ preferences in nutritional values. Fodder samples comprised young leaves, old leaves and young twigs that were taken in July, September and December. It was revealed that nutritive value is not very much influenced by altitude but it is strongly influenced by lopping seasons. Among Thysanolaena maxima, Artocarpus lakoocha, Ficus roxburghii and Bauhinia purpurea the cellulose contents were significantly different (p<0.001) with seasons but there was no significant variation with altitudes. Similarly, Acid Detergent Fibre (ADF) contents in Thysanolaena maxima, Ficus roxburghii and Bauhinia purpurea were found significantly different with seasons. Ficus roxburghii fodder tree was found significantly different in Neutral Detergent Fibre (NDF), ADF, Cellulose and Potassium content with seasons. Similarly, fodder tree Bauhinia purpurea was found significant on Dry matter (DM), Crude fibre (CF), NDF, ADF, cellulose, tannin and potassium with seasons. The study hinted how careful planning of species selection and prudent decision in scheduling looping and feeding fodder trees can help improve animal metabolism, health, growth and productivity

A prospective study on drug utilization pattern & rationality in treatment of type II diabetes mellitus: a population based analysis

Background: Diabetes a chronic disease is associated with significant morbidity, complications with poor glycemic control. Hence, meticulous management is necessary. Comorbid conditions increase chances of polypharmacy and irrational prescriptions in diabetic patients. This prospective non interventional study aimed to gather and study the drug utilization pattern in diabetic patients.Methods: The study was conducted in 50 diabetic patients admitted in medicine ward. Patients were included in the study only after obtaining written informed consent form; all relevant data were collected from case record forms and were analyzed.Results:Diabetes mellitus was observed to be highest in patients with the age group of 60-70 years, affecting 66% males and 34% females. Among the participants 42 (84%) were already on treatment for diabetes while 8 (16%) were diagnosed at the time of admission. We observed that 27 (54%) patients were treated with insulin + oral hypoglycemic agents, 13 (26%) were treated with only Insulin while 10 (20%) patients were prescribed only oral hypoglycemic agents. The most common comorbid conditions observed by us were hypertension, chronic renal disease, diabetic foot, septicemia, urinary tract infections and other susceptible infections.Conclusion:To conclude, the study reveals that Metformin continues to be the choice of oral hypoglycemic agents with least adverse effects and insulin was used to treat uncontrolled state, where physicians have greatly considered the socio-economic status while prescribing which is obvious with least use of costly insulin preparations.

Neuropharmacological evaluation of hormones in mice and insilico analysis of melatonin and somatostatin receptors

Spatial learning and memory consolidation are important aspects of human cognition. The test for motor co-ordination in mice is to characterize the motor phenotype of animals and also in relation to human beings. It is among the most fundamental aspects of everyday life as each physiological process that must be performed in order to achieve movement. In this study, depressive effect and effect on learning and memory of the two hormones, melatonin and somatostatin on animals was evaluated using the loco-motor activity test, rotarod and rectangular maze. The effect of Melatonin and Octreotide acetate alone and in combination with each other were estimated in Swiss albino mice. Melatonin was administered dose dependently and time dependently. Octreotide acetate was administered dose dependently. We compared the combined effect of these hormones for motor co-ordination, loco-motor activity and for the memory and learning behavior of animals. As per the present study, we found significant changes in the motor co-ordination, learning, and memory and depressive effect in animals and there was potentiated depression and attenuated learning and memory when the hormones were used in combination. In-silco analysis was conducted using Patchdock software and molecular docking of the ligands to their respective receptors conducted

Antibacterial Evaluation of Plant Extracts: an insight into Phytomedicine

This study was carried out to evaluate the antibacterial activity of petroleum ether, methanol and aqueous extract of the two plant Ocimum sanctum and pepper nigrum extract using agar well diffusion and broth dilution method against gram-positive bacterial strains (B. firmus, B. megaterium and B. cereus) and gram-negative bacterial strains (Escherichia coli, Enterobacter sp. and Klebsiella pneumoniae). The results indicate that petroleum ether extract compare to methanol and aqueous extract of O. sanctum and P. nigrum exhibited significant antibacterial activity against gram-positive bacteria with minimum inhibitory concentration (MIC) ranging from 0.13 to 0.21x 10-4 mg/well concentration. Moreover, gram-negative bacteria were less susceptible against petroleum ether, methanol and aqueous extract of O. sanctum and P. nigrum and their MIC ranging from 0.13 to 0.21x 10-2. The most susceptible organism to the organic extracts from both studied plants was B. firmus and the most resistant organism was Enterobacter sp. The result obtained with B. cereus and K. pneumoniae were particularly interesting, since it was inhibited by antibiotic ampicillin used and susceptibility was observed with the individual extracts, where higher antibacterial activity with petroleum ether and aqueous extracts of O. sanctum and P. nigrum respectively.  The presence of phytochemicals such as alkaloids, tannins, saponin, triterpenoids, steroids and glycosides in the extracts of these plants supports their traditional uses as medicinal plants for the treatment of various ailments. The present study reveals potential use of these plants for developing new antibacterial herbal drugs against pathogenic microorganisms

Antibacterial Evaluation of Plant Extracts: an insight into Phytomedicine

This study was carried out to evaluate the antibacterial activity of petroleum ether, methanol and aqueous extract of the two plant Ocimum sanctum and pepper nigrum extract using agar well diffusion and broth dilution method against gram-positive bacterial strains (B. firmus, B. megaterium and B. cereus) and gram-negative bacterial strains (Escherichia coli, Enterobacter sp. and Klebsiella pneumoniae). The results indicate that petroleum ether extract compare to methanol and aqueous extract of O. sanctum and P. nigrum exhibited significant antibacterial activity against gram-positive bacteria with minimum inhibitory concentration (MIC) ranging from 0.13 to 0.21x 10-4 mg/well concentration. Moreover, gram-negative bacteria were less susceptible against petroleum ether, methanol and aqueous extract of O. sanctum and P. nigrum and their MIC ranging from 0.13 to 0.21x 10-2. The most susceptible organism to the organic extracts from both studied plants was B. firmus and the most resistant organism was Enterobacter sp. The result obtained with B. cereus and K. pneumoniae were particularly interesting, since it was inhibited by antibiotic ampicillin used and susceptibility was observed with the individual extracts, where higher antibacterial activity with petroleum ether and aqueous extracts of O. sanctum and P. nigrum respectively.  The presence of phytochemicals such as alkaloids, tannins, saponin, triterpenoids, steroids and glycosides in the extracts of these plants supports their traditional uses as medicinal plants for the treatment of various ailments. The present study reveals potential use of these plants for developing new antibacterial herbal drugs against pathogenic microorganisms