The mesolithic occupation at Isolidda (San Vito Lo Capo), Sicily

‘Gruppo dell’Isolidda’ is a complex of five caves along a rocky cliff on the eastern side of the promontory of San Vito Lo Capo (Trapani) in NW Sicily. In 2004 archaeological excavations in the slope below the caves revealed a stratified deposit, partially in secondary position, containing levels with Late Epigravettian and Mesolithic stone tool assemblages. Early Mesolithic stone tool industries, characterized by backed microlithic tools, were distributed in two contiguous layers (SU 21 and SU 25), the lowest of which (SU 21) also contained Epigravettian tools, probably due to sediment reworking. Three AMS dates on Phorcus turbinatus shells (~9520-8990 cal. BP) are chronologically compatible with the Early Mesolithic materials and suggest that the bulk of the deposit accumulated then. A third level, lying above the previous ones, contained material culture associated to the Late Mesolithic or Early Neolithic. Faunal remains from the site represent mainly food refuse and included abundant shells of intertidal molluscs (e.g. Phorcus turbinatus and Patella sp.), along with few fragmented bones of terrestrial herbivores (e.g. Cervus elaphus and Sus scrofa). Oxygen isotope analyses on shell carbonates of Phorcus turbinatus show that, around 9520-9000 cal. BP, marine molluscs were exploited year-round, albeit more often in autumn and winter

Multi-disciplinary Consensus Statement Document Vaccinal prevention in adult patients with diabetes mellitus

open8noopenIcardi G.; Francia F.; Di Bartolo P.; Mannino D.; Alti E.; Purrello F.; Sesti G.; Sessa A.Icardi, G.; Francia, F.; Di Bartolo, P.; Mannino, D.; Alti, E.; Purrello, F.; Sesti, G.; Sessa, A

Prepregnancy BMI influences maternal and fetal outcomes in women with isolated gestational hyperglycaemia: a multicentre study

AIM: This multicentre study analyzed the maternal and fetal outcomes of women who had one elevated 3-h oral glucose tolerance test (isolated gestational hyperglycaemia [IGH]). METHODS: From 1999 to 2003, data were collected for 606 IGH women from 31 Italian obstetric or diabetic centres, including time and mode of delivery, gestational hypertension, preeclampsia, eclampsia, congenital malformations, and neonatal mortality and morbidity, to compare them with the general pregnant Italian population. A prognostic model for the outcome of pregnancy was constructed, and the concurrence of certain specified conditions was considered a positive outcome, whereas pregnancies that failed to meet one or more of the stated conditions were classified as "complicated". RESULTS: Macrosomia was significantly more frequent in women with IGH than in the normal pregnant population (10.7 vs 7.4%, respectively; P=0.003). Stillbirth and neonatal mortality rates did not differ from those in normal pregnancies, while a slight rise in the frequency of major malformations was not statistically significant (1.48 vs 0.89%, respectively; P<0.11). Multivariate logistic analyses confirmed that the prepregnancy body mass index (BMI) was an independent predictor of a complicated pregnancy. As for fetal growth, multivariate logistic analyses according to BMI showed that being overweight or obese were strong predictors of macrosomia. CONCLUSION: These findings in a large cohort of Italian women with IGH confirm the detrimental effect of even minimally altered glucose tolerance on fetal outcome. Also, prepregnancy obesity plays an important role in raising the risk of adverse perinatal outcomes in such patients

INVESTIGATION ON OLD-GROWTH FORESTS OF SICILY: PRELIMINARY RESULTS

Old-growth forests are natural forests that have developed over a long period of time, without experiencing severe, stand-replacing disturbance a fire, windstorm, or logging. According to UNEP/CBD/SBSTTA definition, an old-growth forest is a primary or a secondary forest which has achieved an age at which structure and species normally associated with old primary forests of that type have sufficiently accumulated to act as a forest ecosystem distinct from any younger age class. In a thematic contribution to the National Biodiversity Strategy, the authors report the following definition of Italian old-growth forests: Forest in which human disturbance is absent or negligible, and in which natural dynamics create a mosaic of all the forest regeneration phases, including the senescing one. Such phase is characterized by large old trees, deadwood (snags logs and coarse woody debris) and a vascular plant species composition that is consistent with the biogeographical context and includes highly specialized taxa related to the small-scale disturbance and the microhabitats resulting from structural heterogeneity. In Sicily, 472 hectare were recognized as old-growth forests mainly distributed in Regional Natural Parks, Reserves, Site of Community Importance (SCI) and, Special Protected Areas (SPA). In particular, 18 forest areas, located over 1000 m of altitude and characterized by high index of woodiness, were surveyed in the provinces of Caltanissetta, Catania, Messina and Palermo. In the frame of a research project granted by the University of Palermo, an investigation was carried out in the “Bosco Pomieri”, an old-growth forest included in the Madonie Park (N.-Sicily). In 2013, a multidisciplinary research team started to analyse vascular plant and the cryptogamic diversity, and also carrying out some plant physiology tests and an environmental monitoring. The “Bosco Pomieri” is a mixed oak wood characterized by the presence of many old trees of Quercus petraea (Matt.) Liebl. subsp. austrothyrrenica Brullo, Guarino & Siracusa and a dense shrub layer with Ilex aquifolium L. In the forest area we also find Fagus sylvatica L., Acer pseudoplatanus L., A.obtusatum W. & K., Ulmus glabra Huds., Sorbus torminalis L., Malus sylvestris (L.) Mill., and, sporadically, Quercus cerris L. and Q. pubescens s.l. Shrubby species, such as Daphne laureola L., Crataegus laciniata Ucria, Ruscus aculeatus L. and, Rhamnus catharticus L., are also present in the investigated area (5, 6). Preliminary results of the investigation carried out in the “Bosco Pomieri” are here reported. At present, 9 benthic algal taxa of algae belonging to the Divisions Cyanophyta, Rhodophyta, Chlorophyta, and Bacillariophyta are currently listed. 74 lichens, mainly epiphytic, belonging to the genera Caloplaca Th. Fr., Lecanora Ach., Physcia (Schreb.) Michx. and Ramalina Ach. Fungi are currently represented by 62 saprotrophic, parasitic and mycorrhizal species

Targeting Adenosine Receptor by Polydeoxyribonucleotide: An Effective Therapeutic Strategy to Induce White-to-Brown Adipose Differentiation and to Curb Obesity

Obesity is a worldwide chronic metabolic disease characterized by an abnormal fat accumulation and represents one of the main risk factors for several diseases. White adipose tissue is the primary site for energy storage in the form of triglycerides, whereas brown adipose tissue does not store energy-providing lipids but rather dissipates it by producing heat. White-to-brown adipocyte trans-differentiation could represent a new target of anti-obesity strategies and result in fat reduction. Previous studies indicated that adenosine receptor activation induces trans-differentiation of white adipocytes to brown adipocytes. The aim of this study was to evaluate the effects of polydeoxyribonucleotide (PDRN), an A2Ar receptor agonist, in an in vitro model of browning. Mouse 3T3-L1 pre-adipocytes were differentiated in mature adipocytes with specific culture media and then treated with PDRN (10 µg/mL), PDRN + ZM241385 (1 µM), CGS21680 (1 µM) and CGS + ZM241385 for 24 h. Cell viability was studied by MTT assay, and browning induction was evaluated by Oil Red O staining and by RT-qPCR to study gene expression of browning markers. PDRN, as well as CGS21680, reduced the accumulation of lipids, cell volume and lipid droplet size; increased the expression of UCP1, PRDM16 and DIO2, considered as browning markers; and reduced the expression of FASn and FABP4, considered as whitening markers. In addition, PDRN decreased leptin expression and enhanced adiponectin mRNA levels. All these effects were abrogated when PDRN was co-incubated with the A2Ar antagonist ZM241385. In conclusion, these results suggest that PDRN is able to induce the white-to-brown adipose differentiation through A2Ar stimulation. Since PDRN is a safe drug already available in the market for other therapeutic indications, its “anti-obesity” potential warrants investigation in a clinical scenario

Use of insulin detemir in pregnancy: a report on 10 type 1 diabetic women

Somalia has suffered a massive internal population displacement and exodus that began in 1988 and is still ongoing during the prolonged and intermittent civil war. This review looks at the burden of HIV infection in Somali and the impact of civil war on its epidemiology. Serosurveys have indicated that HIV was not present in Somalia before the civil war and to date Somalia has had an HIV prevalence markedly below that of its neighbours. However, due to the ongoing war HIV sentinel surveillance cannot reach most of the affected areas in Somalia and the current HIV infection problem may be greater than the figures indicate

The Nutraceutical Genistein-Lycopene Combination Improves Bone Damage Induced by Glucocorticoids by Stimulating the Osteoblast Formation Process

Chronic glucocorticoid (GC) therapy is the most common cause of iatrogenic osteoporosis and represents an important risk factor for osteoporosis and bone fractures. New therapeutic approaches are required in order to treat osteoporosis and reduce the side effects related to the use of anti-osteoporotic drugs. In this context, previous studies reported the efficacy of some isoflavones and carotenoids, such as lycopene and genistein, on the reduction of the risk of fracture related to osteoporosis. The aim of this study was to investigate the effects of a combined oral treatment, consisting of genistein and lycopene, in an experimental model of glucocorticoid-induced osteoporosis (GIO). GIO was induced by subcutaneous injection of methylprednisolone (MP, 30 mg/kg) for 60 days, whereas the control group (Sham) received saline solution only. Following induction, MP animals randomly were assigned to receive alendronate, genistein, lycopene, or the association of genistein and lycopene or saline solution for additional 60 days together with MP. Femurs obtained from the Sham group were used for osteoblasts extraction; they were then incubated with dexamethasone (DEX) for 24 h to be then treated with lycopene or genistein or the association of lycopene and genistein for an additional 24 h. Treatments with lycopene and genistein restored the impaired mineralization of cells observed following DEX treatment and stimulated osteoblast differentiation by increasing the depressed expression of bALP and RUNX2 (p < 0.0001). Wnt5a, β-catenin, and Nrf-2 expression were significantly increased following genistein and lycopene treatment (p < 0.0001), thus confirming their antioxidant activity as well as their ability in stimulating osteoblast function, mostly when genistein and lycopene were used in association. The combined treatment of genistein and lycopene improved the bone damage induced by glucocorticoids and significantly restored the normal architecture of bones as well as adequate interconnectivity of bone trabeculae, thus increasing bone mineral density parameters. The obtained data demonstrated that genistein and lycopene but in particular their association might prevent GC’s adverse effects, thus stimulating bone formation and reducing bone resorption, improving bone structure and microarchitecture, through different molecular pathways, such as the Wnt/β-catenin and the Nrf-2 signaling

Mesolithic shellfish exploitation in SW Italy: seasonal evidence from the stable oxygen isotopic composition of /Osilinus turbinatus / shells

Oxygen isotope analyses were carried out on the topshell Osilinus turbinatus from archaeological sites with the aim to investigate the temporal exploitation patterns by Mesolithic groups inhabiting coastal caves in SW Italy. In order to assess the present day O. turbinatus intra-annual