1 research outputs found
Rapid Real-Time Antimicrobial Susceptibility Testing with Electrical Sensing on Plastic Microchips with Printed Electrodes
Rapid antimicrobial
susceptibility testing is important for efficient
and timely therapeutic decision making. Due to globally spread bacterial
resistance, the efficacy of antibiotics is increasingly being impeded.
Conventional antibiotic tests rely on bacterial culture, which is
time-consuming and can lead to potentially inappropriate antibiotic
prescription and up-front broad range of antibiotic use. There is
an urgent need to develop point-of-care platform technologies to rapidly
detect pathogens, identify the right antibiotics, and monitor mutations
to help adjust therapy. Here, we report a biosensor for rapid (<90
min), real time, and label-free bacteria isolation from whole blood
and antibiotic susceptibility testing. Target bacteria are captured
on flexible plastic-based microchips with printed electrodes using
antibodies (30 min), and its electrical response is monitored in the
presence and absence of antibiotics over an hour of incubation time.
We evaluated the microchip with <i>Escherichia coli</i> and
methicillin-resistant <i>Staphylococcus aureus</i> (MRSA)
as clinical models with ampicillin, ciprofloxacin, erythromycin, daptomycin,
gentamicin, and methicillin antibiotics. The results are compared
with the current standard methods, i.e. bacteria viability and conventional
antibiogram assays. The technology presented here has the potential
to provide precise and rapid bacteria screening and guidance in clinical
therapies by identifying the correct antibiotics for pathogens