<i>Pneumocystis</i> carriage in woodmouse: influence of the country of origin and insularity <i>versus</i> mainland (multivariate analysis)^a.

<p>^a OR, odds ratio; CI, confidence interval. Significant results at <i>P</i> = 0.05 country × insularity</p><p><i>Pneumocystis</i> carriage in woodmouse: influence of the country of origin and insularity <i>versus</i> mainland (multivariate analysis)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120839#t002fn001" target="_blank">^a</a>.</p

Minimum spanning network constructed using mtLSU rRNA and mtSSU rRNA combined sequences of <i>Pneumocystis</i> from <i>A</i>. <i>sylvaticus</i>.

<p>Geographic origins (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120839#pone.0120839.t001" target="_blank">Table 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120839#pone.0120839.g001" target="_blank">Fig. 1</a>) are indicated: Spa, Montseny; Pyr, the Pyrenees; Mon, Montpellier; Cal, Calabria; Mal, Mallorca; Min, Minorca; Sic, Sicily; Por, Porquerolles; Cor, Corsica. Numbers correspond to the mutational steps observed between haplotypes.</p

Prevalence of <i>Pneumocystis</i> carriage in woodmice (<i>Apodemus sylvaticus</i>) according to rodent geographic origin.

<p>Woodmice lung DNA samples were screened by nested-PCR at both mtLSU rRNA (LSU) and mtSSU rRNA (SSU) loci for the presence of <i>Pneumocystis</i>. The number of woodmice lung samples positive¹ for <i>Pneumocystis</i> DNA is indicated over the total number of analysed samples. Results of <i>Pneumocystis</i> carriage in wood mice are given according to woodmice geographic origin.</p><p>¹ A wood mice lung sample is considered to be positive for <i>Pneumocystis</i> DNA if the nested-PCR reveals to be positive at either mtLSU rRNA or mtSSU rRNA or both loci.</p><p>^a<i>Pneumocystis</i> concatenated sequences from the samples Cal39 and Cor5 are the same</p><p>^b<i>Pneumocystis</i> concatenated sequences from the samples Spa19, Mon5 and Por15 are the same</p><p>^c<i>Pneumocystis</i> concatenated sequences from the samples Ma47, Ma51 and Ma59 are the same</p><p>^d<i>Pneumocystis</i> concatenated sequences from the samples Ma53, Ma62, Ma66 and Ma71 are the same</p><p>Prevalence of <i>Pneumocystis</i> carriage in woodmice (<i>Apodemus sylvaticus</i>) according to rodent geographic origin.</p

Genetic variability of <i>Pneumocystis</i> concatenated mtLSU and mtSSU sequences observed within each host locality.

<p>GD, genetic divergence; π, nucleotidic diversity; h, haplotype diversity; SD, standard deviation</p><p>Genetic variability of <i>Pneumocystis</i> concatenated mtLSU and mtSSU sequences observed within each host locality.</p

Phylogenetic tree depicting relationships between <i>Pneumocystis</i> organisms isolated from <i>A</i>. <i>sylvaticus</i> of different trapping locations.

<p>NJ, MP, ML and MB are inferred from the combined mitochondrial small subunit (mtSSU) and large subunit (mtLSU) rRNA sequences. The percentages displayed above the branches are the frequencies with which a given branch appeared in 1,000 bootstrap replications in NJ/MP/ML/MB analyses respectively. *Bootstrap values below 50% are depicted by a star.</p

Trapping locations of <i>Apodemus sylvaticus</i>.

<p>Samples are designated according to the location in which they were collected. The number of examined field mice is indicated between brackets. Spa, Montseny (42); Pyr, the Pyrenees (15); Mon, Montpellier (21); Cal, Calabria (39); Mal, Mallorca (25); Min, Minorca (14); Sic, Sicily (32); Por, Porquerolles (10); Cor, Corsica (5). The thick lines correspond to the main European biogeographic barriers: the Pyrenees (west) and the Alps (east). Sample locations are shown by circles.</p